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Chimeric Antisense Oligonucleotides Of Arabinofuranose Analogue And Deoxyribose Nucleotides

Summary

The Present Invention Relates To Novel Oligonucleotide Chimera Used As Therapeutic Agents To Selectively Prevent Gene Transcription And Expression In A Sequence-Specific Manner. In Particular, This Invention Is Directed To The Selective Inhibition Of Protein Biosynthesis Via Antisense Strategy Using Oligonucleotides Constructed From Arabinonucleotide Or Modified Arabinonucleotide Residues, Flanking A Series Of Deoxyribose Nucleotide Residues Of Variable Length. Particularly This Invention Relates To The Use Of Antisense Oligonucleotides Constructed From Arabinonucleotide Or Modified Arabinonucleotide Residues, Flanking A Series Of Deoxyribose Nucleotide Residues Of Variable Length, To Hybridize To Complementary Rna Such As Cellular Messenger Rna, Viral Rna, Etc.; More Particularly This Invention Relates To The Use Of Antisense Oligonucleotides Constructed From Arabinonucleotide Or Modified Arabinonucleotide Residues, Flanking A Series Of Deoxyribose Nucleotide Residues Of Variable Length, To Hybridize To And Induce Cleavage Of (Via Rnaseh Activation) The Complementary Rna.

Supplementary Information

Patent Number: US8178348B2
Application Number: US2003363768A
Inventor: Damha, Masad Jose | Parniak, Michael A. | Lok, Chun-Nam | Vlazovkina, Ekaterina
Priority Date: 6 Sep 2000
Priority Number: US8178348B2
Application Date: 13 Jun 2003
Publication Date: 15 May 2012
IPC Current: C12N000500 | C12N001509 | A61K0031712 | A61K004800 | A61P003112 | A61P004300 | C07H002104 | C12N000502 | C12N0015113
US Class: 435375 | 514044A | 5360232 | 5360245
Assignee Applicant: McGill Universityntreal, Quebec
Title: Chimeric antisense oligonucleotides of arabinofuranose analogue and deoxyribose nucleotides
Usefulness: Chimeric antisense oligonucleotides of arabinofuranose analogue and deoxyribose nucleotides
Summary: (I) is useful for cleaving single stranded RNA, by hybridizing (I) in a sequence-specific manner to a single stranded RNA to induce RNase H activity, and allowing the induced RNase H to cleave the hybridized single stranded RNA. (I) is also useful to prevent translation of single stranded RNA, by hybridizing (I) in a sequence specific manner, to a single stranded RNA, and thus preventing the production of specific protein encoded by the single stranded RNA. (I) is also useful in the preparation of a medicament for cleaving single stranded RNA, e.g. complementary RNA including cellular mRNA or viral RNA. (I) is also useful in the preparation of a probe or laboratory reagent for cleaving single stranded RNA. (All claimed). (I) is useful as a model of antisense oligonucleotide agents, and serves as therapeutics and/or valuable tools for studying and controlling gene expression in cells and organisms.
Novelty: Novel oligonucleotide chimera to selectively prevent gene expression in a sequence-specific manner, comprising a chimera of modified arabinose and 2'-deoxy sugars hybridizing to a single stranded RNA

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

US2003363768A

Country/Region

Hong Kong