METHODS FOR COMPLEX TISSUE ENGINEERING
A simple, highly flexible and scalable platform for making functional complex tissues with heterogeneity and irregularity is provided. The method includes combining undifferentiated cells, such as pluripotent or multipotent stem cells, with a biomaterial to make multiple undifferentiated or naïve subunits, exposing the undifferentiated or naïve subunits to different cell culture environments for induction of differentiation towards different lineages as required by that complex tissue, and combining the then functional subunits with or without the undifferentiated subunits. The differentiated subunits thus combined can be cultured under biological, chemical, and/or physical culture conditions suitable to fine-tune the structural and functional properties of the bioengineered complex tissue to form a bioengineered tissue graft that mimics the structural and functional characteristics of native complex tissue. The bioengineered tissue graft can then used to replace dysfunctional tissue.
Patent Number: US20120148632A1
Application Number: US13159837A
Inventor: Chan, Barbara Pui | Cheng, Hiu Wa | Chik, Daniel Tsz Kit | Cheung, Kenneth Man Chee | Luk, Keith Dip Kei
Priority Date: 15 Jun 2010
Priority Number: US20120148632A1
Application Date: 14 Jun 2011
Publication Date: 14 Jun 2012
IPC Current: A61K000900 | A61K003512 | A61P004300 | C12N000500 | C12N000502 | C12N001100
US Class: 424400 | 4240937 | 435174 | 435325 | 435377
Assignee Applicant: The University of Hong Kong
Title: METHODS FOR COMPLEX TISSUE ENGINEERING
Usefulness: METHODS FOR COMPLEX TISSUE ENGINEERING
Summary: The method (M1) is useful for preparing bioengineered tissue graft used for repairing tissue damage in subject. The method (M2) is useful for producing calcified zone interface in bioengineered tissue construct used for treating osteochondral damage in subject. The method (M3) is useful for producing complex tissue construct. The complex tissue construct is chosen from osteochondral tissue, intervertebral disc, spinal motion segment, ligament bone tissue, cardiac strip with cardiac muscle containing vascularized networks, growth plate with highly organized hierarchical layers of cartilage with bony tissues capable of lengthening and thickening, and liver patch with fully vascularized hepatocytes. The method (M4) is useful for producing bioengineered spinal motion segment (all claimed). The constructs are used in tissue replacement therapies.
Novelty: Preparing bioengineered tissue graft used in tissue replacement therapy, involves combining functional subunits comprising encapsulated pluripotent or multipotent cells induced to differentiate into cell types and culturing subunits
Biomedical
Bioengineering
Hong Kong
