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Wide-dynamic Phase-sensitive Long Range Surface Plasmon Resonance Biosensor with Differential Spectral Interferometry

Summary

Based on research work and patents, the phenomenon of surface Plasmon resonance (SPR) has been applied in optical sensors, especially as a measurement tool for bio-molecular interaction, without the need to label the molecules with techniques such as fluorescence. In contract, the conventional optical transduction technique often requires substantial fluorescence labeling of individual particles or molecules before they could be further processed, thus making it expensive, time consuming and suffering from bleaching after repeated reading. With the growing demands for fact and reliable bio-detection in applications such as medical diagnostics, drug development, forensic investigations and environmental screening, a label-free SPR technique can deliver ultra-fast throughput with uncompromised resolution at the raw material level. This invention develops a wide-dynamic phase-sensitive long-range surface Plasmon resonance (LRSPR) sensor which provides high measurement resolution and broad detection capability through implementing differential spectral interferometry with a wide spectral range light source. The high spectral phase resolution attribute is particularly enhanced when LRSPR is also being incorporated in the sensing layer and the optical platform encompassed a double-passed Michelson interferometer. The invention circumvents two important technical issues, namely, the dynamic range and measurement resolution of current SPR sensors.

Supplementary Information

Inventor: CHAN, HOIYIU KENNETH | HUANG, Wen-zhong | YAM, WINGWAH VIVIAN | YU, Cong
Priority Number: CN101360739B
IPC Current: C07D040114 | C07D040314 | C12Q000168
Assignee Applicant: The University of Hong Kong
Title: Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes | Label-free optical sensing and characterization of biomolecules by d⁸ or d¹⁰ metal complexes
Usefulness: Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes | Label-free optical sensing and characterization of biomolecules by d⁸ or d¹⁰ metal complexes
Summary: For detecting and characterizing a multiply-charged biomolecule, e.g. single-stranded nucleic acid, protein or polypeptide, in a sample (claimed). Also useful for detecting structural changes of target single-stranded nucleic acid and detecting mutation in a target single-stranded nucleic acid.
Novelty: Composition for detecting charged biomolecule comprises charged metal complex with 8 or 10 electrons in d-orbital, which electrostatically binds to biomolecule to induce aggregation and self-assembly via metal-metal and pi-pi interactions

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

10/ENG/360

Others

Inventor(s): Professor Aaron HO, Department of Electronic Engineering

Patent Status: US Patent Pending Licensing Status: Available for licensing

Country/Region

Hong Kong