A-45 A Novel Screening Method for Anti-malarial Compounds
ΓùÅ very unique DNA polymerase which is heat-resistant and requires Fe2+, not Mg2+, for its activationΓùÅ the heat-inactivation of mtDNA polymerase doesnΓÇÖt occur over 90 ΓäâΓùÅ mtDNA polymerase has been proved with cell-free protein synthesis
Could be used as a screening method for new antimalarial medicines
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Inventor: SASAKI Narie | MUROFUSHI Kimiko | MAEDA Katsura
Priority Number: WO2012002180A1
IPC Current: C12Q000168 | C12Q000148 | C12N001509
Assignee Applicant: National University Corporation Nagoya University
Title: METHOD FOR DETERMINATION OF ACTIVITY OF MITOCHONDRIAL DNA POLYMERASE OF PLASMODIUM FALCIPARUM MALARIA, AND METHOD FOR SCREENING FOR ANTI-MALARIA COMPOUND | PROCÉDÉ DE DÉTERMINATION D'ACTIVITÉ D'ADN POLYMÉRASE MITOCHONDRIALE DU PALUDISME À PLASMODIUM FALCI
Usefulness: METHOD FOR DETERMINATION OF ACTIVITY OF MITOCHONDRIAL DNA POLYMERASE OF PLASMODIUM FALCIPARUM MALARIA, AND METHOD FOR SCREENING FOR ANTI-MALARIA COMPOUND | PROCÉDÉ DE DÉTERMINATION D'ACTIVITÉ D'ADN POLYMÉRASE MITOCHONDRIALE DU PALUDISME À PLASMODIUM FALCIPARUM, ET PROCÉDÉ DE CRIBLAGE DE COMPOSÉ CONTRE LE PALUDISME
Summary: The method (M1) is useful for measuring DNA polymerase activity. The method (M2) is useful for screening antimalarial compound (claimed).
Novelty: Measuring DNA polymerase activity, by incubating solution containing bivalent iron ion, mitochondrial DNA polymerase and template DNA, detecting synthesized double-stranded DNA, and computing activity of DNA polymerase
生物醫學
DNA /基因工程
美國
