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A-45 A Novel Screening Method for Anti-malarial Compounds

技术优势

ΓùÅ very unique DNA polymerase which is heat-resistant and requires Fe2+, not Mg2+, for its activationΓùÅ the heat-inactivation of mtDNA polymerase doesnΓÇÖt occur over 90 ΓäâΓùÅ mtDNA polymerase has been proved with cell-free protein synthesis

技术应用

Could be used as a screening method for new antimalarial medicines

详细技术说明

None

*Abstract

Nagoya University researchers have found that mitochondrial DNA polymerase (mtDNA polymerase) of plasmodium faciparum malaria requires a bivalent iron ion (Fe2+) for its activity and that it is heat-resistant and most active around 70 Γäâ. Researchers established the method to calculate the activity of mtDNA polymerase by using Fe2+, mtDNA polymerase, template DNA, and dNTP. This method could be used to screen new compounds to identify potential anti-malarial drug that inhibits DNA synthesis.

附加资料

Inventor: SASAKI Narie | MUROFUSHI Kimiko | MAEDA Katsura
Priority Number: WO2012002180A1
IPC Current: C12Q000168 | C12Q000148 | C12N001509
Assignee Applicant: National University Corporation Nagoya University
Title: METHOD FOR DETERMINATION OF ACTIVITY OF MITOCHONDRIAL DNA POLYMERASE OF PLASMODIUM FALCIPARUM MALARIA, AND METHOD FOR SCREENING FOR ANTI-MALARIA COMPOUND | PROCÉDÉ DE DÉTERMINATION D'ACTIVITÉ D'ADN POLYMÉRASE MITOCHONDRIALE DU PALUDISME À PLASMODIUM FALCI
Usefulness: METHOD FOR DETERMINATION OF ACTIVITY OF MITOCHONDRIAL DNA POLYMERASE OF PLASMODIUM FALCIPARUM MALARIA, AND METHOD FOR SCREENING FOR ANTI-MALARIA COMPOUND | PROCÉDÉ DE DÉTERMINATION D'ACTIVITÉ D'ADN POLYMÉRASE MITOCHONDRIALE DU PALUDISME À PLASMODIUM FALCIPARUM, ET PROCÉDÉ DE CRIBLAGE DE COMPOSÉ CONTRE LE PALUDISME
Summary: The method (M1) is useful for measuring DNA polymerase activity. The method (M2) is useful for screening antimalarial compound (claimed).
Novelty: Measuring DNA polymerase activity, by incubating solution containing bivalent iron ion, mitochondrial DNA polymerase and template DNA, detecting synthesized double-stranded DNA, and computing activity of DNA polymerase

主要类别

生物医学

细分类别

DNA /基因工程

国家/地区

美国