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Monoclonal Antibody Panel Against Non-Structural Protein (NS) of Tomato Spotted Wilt Virus (TSWV, Tospovirus) (4 lines)

Detailed Technology Description: Applications Suitable for DAS-ELISA, PAS-ELISA and DIBA applications. Tomato spotted wilt tospovirus (TSWV) is the type member ofthe plant-infecting viruses of the genus Tospovirus in the family Bunyaviridae.TSWV is transmitted by thrips (Thysanoptera: Thripidae) to over 650 differentplant species in temperate and tropical regions. Once the plants infected withthe virus, they result in spotting, wilting, reduced vegetative output anddeath of the plant. Some of the crops known to be affected by the virusesinclude, peanuts, watermelons, tomatoes, zucchinis and others.

*Abstract

ReagentsDescription

Clone Name	Antigen	Isotype
1C1.A3 HL1	Non-structural protein encoded by the small RNA of TSWV	IgG2a
1C1.A7 HL1	Non-structural protein encoded by the small RNA of TSWV	IgG2a
1C1.A7	Non-structural protein encoded by the small RNA of TSWV	IgG2a
1C3.A5	Non-structural protein encoded by the small RNA of TSWV	IgG2a

Testedapplications: Western Blot, ELISA, ACP-ELISA and DAS-ELISA applications

Antigen:Non-structural protein encoded by the small RNA of TSWV

Reactivity: TSWV, possibly other Tospoviruses

SpeciesImmunized: Mouse

PurificationMethod: Protein G

Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15MNaCl, 0.05% (w/v) Sodium Azide

Antibodystorage temperature: -80^oC

References

Bandla, MD,Daphne, MW; Chenault, K.D.; Ullman, DE; German, TL; Sherwood, JL: Use ofMonoclonal Antibody to the Nonstructural Protein Encoded by the Small RNA ofTomato Spotted Wilt Tospovirus to Identify Viruliferous Thirps: Phytopathology,84(12), 1427-1431 (1994).

Naidu, RA;Sherwood, JL; Deom, CM. Characterization of a vector-non-transmissible isolateof Tomato spotted wilt virus. Plant Pathol,57, 190-200 (2008).

Sherwood, JL,Sanborn, MR, Keyser, GC; Myers, LD. Use of Monoclonal Antibodies in Detectionof Tomato Spotted Wilt Virus, Phytopathology,79(1), 61-64 (1989).

Richmond, KE,Chenault, K; Sherwood, JL; Geramn, TL: Characterization of the Nucleic AcidBinding Properties of Tomato Spotted Wilt Virus Nucleocapsid Protein, Virology 248,6-11 (1998).

Adkins,S, Choi, T-J,Israel, BA, Bandla, MD, Richmond, KE, Schultz, KT, Sherwood, JL,German, TL. Baculovirus Expression and Processing of Tomato Spotted WiltTopovirus Glycoproteins. Phytopathology86(8), 849-855 (1996).

Country/Region: USA

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