Monoclonal Antibody Panel Against Non-Structural Protein (NS) of Tomato Spotted Wilt Virus (TSWV, Tospovirus) (4 lines)
- 详细技术说明
- Applications Suitable for DAS-ELISA, PAS-ELISA and DIBA applications. Tomato spotted wilt tospovirus (TSWV) is the type member ofthe plant-infecting viruses of the genus Tospovirus in the family Bunyaviridae.TSWV is transmitted by thrips (Thysanoptera: Thripidae) to over 650 differentplant species in temperate and tropical regions. Once the plants infected withthe virus, they result in spotting, wilting, reduced vegetative output anddeath of the plant. Some of the crops known to be affected by the virusesinclude, peanuts, watermelons, tomatoes, zucchinis and others.
- *Abstract
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ReagentsDescription
Clone Name
Antigen
Isotype
1C1.A3 HL1
Non-structural protein encoded by the small RNA of TSWV
IgG2a
1C1.A7 HL1
Non-structural protein encoded by the small RNA of TSWV
IgG2a
1C1.A7
Non-structural protein encoded by the small RNA of TSWV
IgG2a
1C3.A5
Non-structural protein encoded by the small RNA of TSWV
IgG2a
Testedapplications: Western Blot, ELISA, ACP-ELISA and DAS-ELISA applications
Antigen:Non-structural protein encoded by the small RNA of TSWV
Reactivity: TSWV, possibly other Tospoviruses
SpeciesImmunized: Mouse
PurificationMethod: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15MNaCl, 0.05% (w/v) Sodium Azide
Antibodystorage temperature: -80oC
References
Bandla, MD,Daphne, MW; Chenault, K.D.; Ullman, DE; German, TL; Sherwood, JL: Use ofMonoclonal Antibody to the Nonstructural Protein Encoded by the Small RNA ofTomato Spotted Wilt Tospovirus to Identify Viruliferous Thirps: Phytopathology,84(12), 1427-1431 (1994).
Naidu, RA;Sherwood, JL; Deom, CM. Characterization of a vector-non-transmissible isolateof Tomato spotted wilt virus. Plant Pathol,57, 190-200 (2008).
Sherwood, JL,Sanborn, MR, Keyser, GC; Myers, LD. Use of Monoclonal Antibodies in Detectionof Tomato Spotted Wilt Virus, Phytopathology,79(1), 61-64 (1989).
Richmond, KE,Chenault, K; Sherwood, JL; Geramn, TL: Characterization of the Nucleic AcidBinding Properties of Tomato Spotted Wilt Virus Nucleocapsid Protein, Virology 248,6-11 (1998).
Adkins,S, Choi, T-J,Israel, BA, Bandla, MD, Richmond, KE, Schultz, KT, Sherwood, JL,German, TL. Baculovirus Expression and Processing of Tomato Spotted WiltTopovirus Glycoproteins. Phytopathology86(8), 849-855 (1996).
- 国家/地区
- 美国
