Trypanosoma cruzi CRISPR/Cas9 System Plasmid, PUC_sgRNA
- Detailed Technology Description
- Applications Contains the empty sgRNA backbone sequence (tracrRNA, 82 bp)and is used as DNA template to amplify a specific sgRNA using a forward primerwith the protospacer sequence for gene targeting. Trypanosoma cruzi CRISPR/Cas9 System plasmids allow for theamplification of a specific sgRNA sequence or express cas9 to generateCRISPR-ablated, red/green fluorescent parasites. Trypanosomacruziis the agent of Chagas disease, which affects millions of people worldwide.Vaccines to prevent this disease are not available, and drug treatments are notcompletely effective. The study of the biology of this parasite through geneticapproaches will make possible the development of new preventive or treatmentoptions.
- *Abstract
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Reagent Description
Gene/Insert Name: tracrRNA sequence
Insert Size (bp): 82 bp
Species: N/A
Fusion Proteins/Tags: N/A
Vector Backbone and Size (bp): pUCAmp; 3150 bp
Cloning Site 5’: 5′ sequencing primer: M13_forward20_primer and M13_pUC_fwd_primer
Cloning Site 3’: 3′ sequencing primer: M13_reverse_primer and M13_pUC_rev_primer
Antibiotic Resistance: Ampicillin
High or Low Copy: High
Grow in Standard E. coli @ 37oC? DH5alpha; 37°C
Selectable Markers: Neomycin (select with G418)
Recommended Storage Temperature: -20oC
References
LanderN, Li ZH, Niyogi S, Docampo R. CRISPR/Cas9-Induced Disruption of ParaflagellarRod Protein 1 and 2 Genes in Trypanosomacruzi Reveals Their Role in Flagellar Attachment. mBIO. 2015.6(4): e01012-15.
- Country/Region
- USA
