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Trypanosoma cruzi CRISPR/Cas9 System Plasmid, PUC_sgRNA
详细技术说明
Applications Contains the empty sgRNA backbone sequence (tracrRNA, 82 bp)and is used as DNA template to amplify a specific sgRNA using a forward primerwith the protospacer sequence for gene targeting. Trypanosoma cruzi CRISPR/Cas9 System plasmids allow for theamplification of a specific sgRNA sequence or express cas9 to generateCRISPR-ablated, red/green fluorescent parasites. Trypanosomacruziis the agent of Chagas disease, which affects millions of people worldwide.Vaccines to prevent this disease are not available, and drug treatments are notcompletely effective. The study of the biology of this parasite through geneticapproaches will make possible the development of new preventive or treatmentoptions.
国家/地区
美国
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