In-Vivo Quantitative Fluorescence Probe for Surgical Guidance and "Smart" Biopsies
- Technology Application
- Identification of thelast remaining tumor cells during surgery to maximize resection completenessand extend patient’s lifePrior to biopsy, tissue fluorescence can be measured to confirm that thebiopsy is likely to contain tumor cells
- Detailed Technology Description
- Handheld probe quantitatively identifies tumor cellsin vivo, for needle biopsies and guiding surgical interventions/resections
- *Abstract
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UHN scientists have developed a unique handheldprobe that can quantitatively measure tissue fluorescence in vivo; such fluorescence can be elicited in tumor cells with theaid of an orally given drug. The key differentiator from other quantitativefluorescence devices is its small handheld size and the important ability tocompensate/correct for the dramatic effects of tissue optical propertyvariation on fluorescence readings, providing the true, quantitative assessmentof fluorophore-tagged pathologic tissue. This is important for the purpose ofproviding the surgeon with useful real-time information to determine thepresence of malignancy at the site of interest. Clinical trials are underwayfor testing of this probe’s ability to find the last few invisible tumor cellsleftover in brain tumor resection surgery, with early data showing that theprobe is far more accurate compared with a surgeon’s visual determination.
The probe has been further integrated into a biopsydevice for guidance of tissue biopsy procedures. By integrating this probe intoa biopsy needle, the surgeon can identify tumor cells prior to acquiring thetissue, leading to faster and fewer repeated procedures.The quantitative fluorescence probe is portable, sterilizable and can be integrated into the surgical workflow. It is being used in neurosurgical trials for brain cancer removal. For high-grade glioma, the probe data has proven to be dramatically more sensitive and specific than both the surgeon’s visual assessment and an uncorrected fluorescence signal that represents the current state-of-the-art detection techniques (probe data is compared against the gold standard of tissue biopsy).
- *Principal Investigator
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Dr. Brian C. Wilson, University Health Network
- *Publications
- Kim A, Khurana M, Moriyama Y, Wilson BC. Quantification of in vivo fluorescence decoupledfrom the effects of tissue optical properties using fiber-optic spectroscopymeasurements. J Biomed Opt. 2010;15(6):067006.Valdés PA, Leblond F, Kim A, Harris BT, Wilson BC,Fan X, Tosteson TD, Hartov A, Ji S, Erkmen K, Simmons NE, Paulsen KD, RobertsDW. Quantitative fluorescencein intracranial tumor: implications for ALA-induced PpIX as an intraoperativebiomarker.J Neurosurg.2011;115(1):11-7ValdesPA, Bekelis K, Harris BT, Wilson BC, Leblond F, Kim A, Simmons NE, Erkmen K,Paulsen KD, Roberts DW.5-Aminolevulinic acid-induced protoporphyrin IX fluorescence in meningioma:qualitative & quantitative measurements in vivo.Neurosurgery. 2014;10 Suppl 1:74-82.
- Country/Region
- USA
