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Cloned Zebrafish from Long-Term Cultured Cells

Technology Benefits

Long-term-cultured cells to provide adequate time for use in targeted genetic manipulation

Technology Application

Clone fertile zebrafish from long-term cell cultures to allow for targeted genetic manipulation

Detailed Technology Description

UCLA researchers have demonstrated the successful cloning of fertile zebrafish from long-term-cultured cells. Transgenic zebrafish are obtained by nuclear transfer using embryonic fibroblast cells from long-term cultures. These transgenic zebrafish will allow the research community to work on targeted genetic manipulation in zebrafish.

Supplementary Information

Patent Number: US7332647B2
Application Number: US2003517880A
Inventor: Lin, Shuo
Priority Date: 11 Jun 2002
Priority Number: US7332647B2
Application Date: 13 Dec 2004
Publication Date: 19 Feb 2008
IPC Current: A01K0067027 | C12N001500 | C12N0015873
US Class: 800020 | 800024
Assignee Applicant: The Regents of the University of California
Title: Fish produced by nuclear transfer from cultured cells
Usefulness: Fish produced by nuclear transfer from cultured cells
Summary: The methods are useful for making a diploid transgenic fish or a progeny fish (claimed).

Industry

Agriculture

Sub Category

Plant

Application No.

7332647

Others

State Of Development

Inventors have successfully developed and tested the cloning procedure for transgenic zebrafish from long-term-cultured cells.

Background

Although mammals have been cloned from genetically manipulated cultured cells, there is still a need for lower vertebrates to be used. Since zebrafish are important lower vertebrates to use for the study of vertebrate development, cloned zebrafish may offer an alternative to embryonic stem cells. Zebrafish cell cultures exhibit some characteristics of embryonic stem cells and are easier to use in research involving genetic manipulation. However, only short-term cell cultures of zebrafish exist for the study of vertebrate development.

Related Materials

Lee K, Huang H, Ju B, Yang Z, Lin S. "Cloned zebrafish by nuclear transfer from long-term-cultured cells". Nature Biotechnology. 20, 795-799 (2002)

Tech ID/UC Case

22293/2002-491-0

Related Cases

2002-491-0

*Abstract

UCLA investigators have successfully demonstrated the cloning of transgenic zebrafish from long-term-cultured cells to provide adequate time for use in targeted genetic manipulation. Zebrafish also exhibit characteristics of embryonic stem cells.

*Applications

Clone fertile zebrafish from long-term cell cultures to allow for targeted genetic manipulation

*IP Issue Date

Feb 19, 2008

*Principal Investigator

Name: Shuo Lin

Department:

Country/Region

USA