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Proteins that Efficiently Generate Singlet Oxygen Background

技術優勢

Significantly higher quantum yields than current system.No exogenous co-factors needed.Provides a new level of detection and utility for proteomic studies utilizing: Electron microscopy and fluorescent imaging.Chromophore assisted light inactivation for functional proteomics.Greater monitoring of protein-protein distances than FRET based sensors that is independent of chromophore orientation.

詳細技術說明

UC San Diego researchers have developed a fully genetically encoded singlet oxygen system called mini-SOG. The system uses truncated proteins that can be fused to a protein of interest and expressed in either bacterial or mammalian cells. Although requiring flavin mononucleotide (FMN) as a co-factor, it is endogenously available to cells, and because it is a derivative of riboflavin, no exogenous addition is necessary. The FMN holoprotein will fluoresce upon light excitation and generates singlet oxygen with quantum yields in excess of 0.4.

附加資料

Patent Number: US20130330718A1
Application Number: US13992540A
Inventor: Shu, Xiaokun | Tsien, Roger Y.
Priority Date: 7 Dec 2010
Priority Number: US20130330718A1
Application Date: 20 Aug 2013
Publication Date: 12 Dec 2013
IPC Current: G01N002164 | C07K001400
US Class: 4350061 | 4352523 | 43525233 | 4353201 | 530350 | 5360231
Assignee Applicant: The Regents of the University of California
Title: PROTEINS THAT EFFICIENTLY GENERATE SINGLET OXYGEN
Usefulness: PROTEINS THAT EFFICIENTLY GENERATE SINGLET OXYGEN
Summary: In in vivo imaging method; for generating singlet oxygen in a cell and irradiating the cell with blue light; for monitoring photodynamic therapy in a subject; for engineering miniSOG protein (all claimed); in optical and electron microscope imaging, detection and localization of protein-protein interaction and photoblation.
Novelty: New isolated polynucleotide encoding mini singlet oxygen generator polypeptide of light, oxygen, and voltage domain of mutation at conserved cysteine corresponding to specific position of specific amino acid sequence for in vivo imaging