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Proteins that Efficiently Generate Singlet Oxygen Background

Technology Benefits

Significantly higher quantum yields than current system.No exogenous co-factors needed.Provides a new level of detection and utility for proteomic studies utilizing: Electron microscopy and fluorescent imaging.Chromophore assisted light inactivation for functional proteomics.Greater monitoring of protein-protein distances than FRET based sensors that is independent of chromophore orientation.

Detailed Technology Description

UC San Diego researchers have developed a fully genetically encoded singlet oxygen system called mini-SOG. The system uses truncated proteins that can be fused to a protein of interest and expressed in either bacterial or mammalian cells. Although requiring flavin mononucleotide (FMN) as a co-factor, it is endogenously available to cells, and because it is a derivative of riboflavin, no exogenous addition is necessary. The FMN holoprotein will fluoresce upon light excitation and generates singlet oxygen with quantum yields in excess of 0.4.

Supplementary Information

Patent Number: US20130330718A1
Application Number: US13992540A
Inventor: Shu, Xiaokun | Tsien, Roger Y.
Priority Date: 7 Dec 2010
Priority Number: US20130330718A1
Application Date: 20 Aug 2013
Publication Date: 12 Dec 2013
IPC Current: G01N002164 | C07K001400
US Class: 4350061 | 4352523 | 43525233 | 4353201 | 530350 | 5360231
Assignee Applicant: The Regents of the University of California
Title: PROTEINS THAT EFFICIENTLY GENERATE SINGLET OXYGEN
Usefulness: PROTEINS THAT EFFICIENTLY GENERATE SINGLET OXYGEN
Summary: In in vivo imaging method; for generating singlet oxygen in a cell and irradiating the cell with blue light; for monitoring photodynamic therapy in a subject; for engineering miniSOG protein (all claimed); in optical and electron microscope imaging, detection and localization of protein-protein interaction and photoblation.
Novelty: New isolated polynucleotide encoding mini singlet oxygen generator polypeptide of light, oxygen, and voltage domain of mutation at conserved cysteine corresponding to specific position of specific amino acid sequence for in vivo imaging

Industry

Biomedical

Sub Category

Medical Imaging

Application No.

20130330718

Others

Intellectual Property Info

A provisional patent application has been filed.

Related Materials

Abstract presented at the 54th Annual Biophysical Society Meeting, February 20-24, 2010. Biophysical Journal, Volume 98, Issue 3, Supplement 1, January 2010, Page 612a.

Additional Technologies by these Inventors

• Molecules for Labeling Peripheral Nerves for use in Image Guided Surgery and Other Clinical Applications
• Proteins That Fluoresce At Infrared Wavelengths Or Singlet Oxygen Upon Illumination
• Dual Reflectance-Fluorescence Guided Surgical System
• Personalized Protease fingerprinting for early cancer diagnosis

Tech ID/UC Case

20715/2010-049-0

Related Cases

2010-049-0

*Abstract

Singlet oxygen generation is a well-known technique in proteomic studies that involve localization of proteins by electron microscopy, inactivation of proteins for functional studies, and measurement of the distances between interacting proteins.

One of the most widely used reagent systems is the biarsenical ReAsh-Tetracysteine system from Life Technologies. While partially genetically encoded, it requires the cells to be stimulated to overexpress a needed cofactor and generate singlet oxygen with a quantum yield of less than 0.05. Attempts to develop other systems have not resulted in higher quantum yields and, in some cases while resulting in fluorescence, have not been shown to produce singlet oxygen.

*IP Issue Date

Dec 12, 2013

*Principal Investigator

Name: Xiaokun Shu

Department:

Name: Roger Tsien

Department:

Country/Region

USA