Chemical Sensing by RIFTS-Reflective Interferometric Fourier-Transform Spectroscopy: A Robust, Self-Compensating Method for Label-Free Detection of Biomolecules
The approach is very general. For example, the methodology should also work with other label-free transduction modalities in materials other than porous SiO 2 or porous Si that utilize refractive index changes, such as surface plasmon resonance or microcavity resonance. The built-in reference channel and Fourier method of analysis provides a general means to compensate for changes in sample matrix, non-specific binding, temperature, and other experimental variables.
Label-free biosensing, high-throughput molecular sensing, array-based sensing, drug lead discovery, diagnostics, and characterization of kinetic and thermodynamic binding constants in biomolecular binding assays.
This invention utilizes a novel self-compensating interferometric biosensor comprised of two layers of porous SiO 2, stacked one on top of the other. The reflectivity spectrum displays a complex interference pattern that arises from a combination of Fabry-Pérot interference from these layers. A ratio of the peak intensities in the fast Fourier transform (FFT) allows discrimination of target analyte from matrix effects arising from non-specific compositional changes in the analyte solution.
Patent Number: US20110170106A1
Application Number: US2010683895A
Inventor: Pacholski, Claudia | Miskelly, Gordon M. | Sailor, Michael J.
Priority Date: 10 Mar 2005
Priority Number: US20110170106A1
Application Date: 7 Jan 2010
Publication Date: 14 Jul 2011
IPC Current: G01J000345
US Class: 356451
Assignee Applicant: The Regents of the University of California
Title: MULTIPLE SUPERIMPOSED INTERFACE PATTERN POROUS MICROSTRUCTURE MULTI LAYER BIOSENSING METHOD
Usefulness: MULTIPLE SUPERIMPOSED INTERFACE PATTERN POROUS MICROSTRUCTURE MULTI LAYER BIOSENSING METHOD
Summary: Porous microstructure multilayer bio-sensing method.
Novelty: Porous microstructure multilayer bio-sensing method, involves biological analyte to multilayer micro-porous thin film structure, and determining presence of bio molecule of interest in analyte from extracted optical parameters
生物醫學
醫療裝置
9909985
State Of Development The concept has been demonstrated with a Protein A capture probe and Human Immunoglobulin G as the target analyte. The system response is shown to be insensitive to the addition of 4000-fold excess sucrose or 80-fold excess bovine serum albumin. Related Materials Intellectual Property Info A patent application is pending. Related Materials Létant, S. & Sailor, M. J. Molecular Identification by Time Resolved Interferometry in a Porous Silicon Film. Adv. Mat. 13, 335-338 (2001). Tech ID/UC Case 19503/2005-179-0 Related Cases 2005-179-0
Ghadiri, M. R., Sailor, M. J., Motesharei, K., Lin, S.-Y. & Dancil, K.-P. S. Porous semiconductor-based optical interferometric sensor. See U.S. patent number 6,720,177.
Ghadiri, M. R., Sailor, M. J., Motesharei, K., Lin, S.-Y. & Dancil, K.-P. S. Porous semiconductor-based optical interferometric sensor. See U.S. patent number 6,897,965.
Ghadiri, M. R., Motesharei, K., Lin, S.-Y., Sailor, M. J. & Dancil, K.-P. Porous semiconductor-based optical interferometric sensor. See U.S. patent number 6,248,539.
Sohn, H., Létant, S., Sailor, M. J. & Trogler, W. C. Detection of Fluorophosphonate Chemical Warfare Agents by Catalytic Hydrolysis with a Porous Silicon Interferometer. J. Am. Chem. Soc. 122, 5399-5400 (2000).
Dancil, K.-P. S., Greiner, D. P. & Sailor, M. J. A Porous Silicon Optical Biosensor: Detection of Reversible Binding of IgG to a Protein A-Modified Surface. J. Am. Chem. Soc. 121, 7925-7930 (1999).
Janshoff, A. et al. Macroporous P-Type Silicon Fabry-Perot Layers. Fabrication, Characterization, and Applications in Biosensing. J. Am. Chem. Soc. 120, 12108-12116 (1998).
Lin, V. S.-Y., Motesharei, K., Dancil, K. S., Sailor, M. J. & Ghadiri, M. R. A Porous Silicon-Based Optical Interferometric Biosensor. Science 278, 840-843 (1997).
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