Chemical Sensing by RIFTS-Reflective Interferometric Fourier-Transform Spectroscopy: A Robust, Self-Compensating Method for Label-Free Detection of Biomolecules
The approach is very general. For example, the methodology should also work with other label-free transduction modalities in materials other than porous SiO 2 or porous Si that utilize refractive index changes, such as surface plasmon resonance or microcavity resonance. The built-in reference channel and Fourier method of analysis provides a general means to compensate for changes in sample matrix, non-specific binding, temperature, and other experimental variables.
Label-free biosensing, high-throughput molecular sensing, array-based sensing, drug lead discovery, diagnostics, and characterization of kinetic and thermodynamic binding constants in biomolecular binding assays.
This invention utilizes a novel self-compensating interferometric biosensor comprised of two layers of porous SiO 2, stacked one on top of the other. The reflectivity spectrum displays a complex interference pattern that arises from a combination of Fabry-Pérot interference from these layers. A ratio of the peak intensities in the fast Fourier transform (FFT) allows discrimination of target analyte from matrix effects arising from non-specific compositional changes in the analyte solution.
Patent Number: US20110170106A1
Application Number: US2010683895A
Inventor: Pacholski, Claudia | Miskelly, Gordon M. | Sailor, Michael J.
Priority Date: 10 Mar 2005
Priority Number: US20110170106A1
Application Date: 7 Jan 2010
Publication Date: 14 Jul 2011
IPC Current: G01J000345
US Class: 356451
Assignee Applicant: The Regents of the University of California
Title: MULTIPLE SUPERIMPOSED INTERFACE PATTERN POROUS MICROSTRUCTURE MULTI LAYER BIOSENSING METHOD
Usefulness: MULTIPLE SUPERIMPOSED INTERFACE PATTERN POROUS MICROSTRUCTURE MULTI LAYER BIOSENSING METHOD
Summary: Porous microstructure multilayer bio-sensing method.
Novelty: Porous microstructure multilayer bio-sensing method, involves biological analyte to multilayer micro-porous thin film structure, and determining presence of bio molecule of interest in analyte from extracted optical parameters
Biomedical
Medical Device
9909985
State Of Development The concept has been demonstrated with a Protein A capture probe and Human Immunoglobulin G as the target analyte. The system response is shown to be insensitive to the addition of 4000-fold excess sucrose or 80-fold excess bovine serum albumin. Related Materials Intellectual Property Info A patent application is pending. Related Materials Létant, S. & Sailor, M. J. Molecular Identification by Time Resolved Interferometry in a Porous Silicon Film. Adv. Mat. 13, 335-338 (2001). Tech ID/UC Case 19503/2005-179-0 Related Cases 2005-179-0
Ghadiri, M. R., Sailor, M. J., Motesharei, K., Lin, S.-Y. & Dancil, K.-P. S. Porous semiconductor-based optical interferometric sensor. See U.S. patent number 6,720,177.
Ghadiri, M. R., Sailor, M. J., Motesharei, K., Lin, S.-Y. & Dancil, K.-P. S. Porous semiconductor-based optical interferometric sensor. See U.S. patent number 6,897,965.
Ghadiri, M. R., Motesharei, K., Lin, S.-Y., Sailor, M. J. & Dancil, K.-P. Porous semiconductor-based optical interferometric sensor. See U.S. patent number 6,248,539.
Sohn, H., Létant, S., Sailor, M. J. & Trogler, W. C. Detection of Fluorophosphonate Chemical Warfare Agents by Catalytic Hydrolysis with a Porous Silicon Interferometer. J. Am. Chem. Soc. 122, 5399-5400 (2000).
Dancil, K.-P. S., Greiner, D. P. & Sailor, M. J. A Porous Silicon Optical Biosensor: Detection of Reversible Binding of IgG to a Protein A-Modified Surface. J. Am. Chem. Soc. 121, 7925-7930 (1999).
Janshoff, A. et al. Macroporous P-Type Silicon Fabry-Perot Layers. Fabrication, Characterization, and Applications in Biosensing. J. Am. Chem. Soc. 120, 12108-12116 (1998).
Lin, V. S.-Y., Motesharei, K., Dancil, K. S., Sailor, M. J. & Ghadiri, M. R. A Porous Silicon-Based Optical Interferometric Biosensor. Science 278, 840-843 (1997).
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