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Trypanosoma cruzi CRISPR/Cas9 System Plasmid, CAS9/pTREX-n
详细技术说明
Applications This plasmid expresses the fusion gene CAS9-HA-2xNS-GFP (S. pyogenes Cas9 sequence with atwice-repeated sequence of the simian virus 40 nuclear localization signal andGFP) in the pTREX-n backbone. This vector is used for cloning a specific sgRNAby BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi. Trypanosomacruziis the agent of Chagas disease, which affects millions of people worldwide.Vaccines to prevent this disease are not available, and drug treatments are notcompletely effective. The study of the biology of this parasite through geneticapproaches will make possible the development of new preventive or treatmentoptions.
国家/地区
美国
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