Trypanosoma cruzi CRISPR/Cas9 System Plasmid, CAS9/pTREX-n
- Detailed Technology Description
- Applications This plasmid expresses the fusion gene CAS9-HA-2xNS-GFP (S. pyogenes Cas9 sequence with atwice-repeated sequence of the simian virus 40 nuclear localization signal andGFP) in the pTREX-n backbone. This vector is used for cloning a specific sgRNAby BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi. Trypanosomacruziis the agent of Chagas disease, which affects millions of people worldwide.Vaccines to prevent this disease are not available, and drug treatments are notcompletely effective. The study of the biology of this parasite through geneticapproaches will make possible the development of new preventive or treatmentoptions.
- *Abstract
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Reagent Description
Name: CAS9/pTREX-n
Gene/insert name: Fusion gene CAS9-HA-2xNLS-GFP
Fusion Tag(s): Fusion gene Cas9-HA-2xNLS-GFP
Antibiotic Resistance: Ampicillin
Grow in E. coli at 37 C: DH5alpha; 37C
Selectable markers: Neomycin (select with G418)
Cloning Site 5': XbaI
Cloning Site 3': HindIII
Insert Size: 4975 bp
Vector Backbone and Size: pTREX-n, backbone size without insert: 6227bp
High or low copy: High
Storage: -20C
Gene/Insert Name: Fusion gene CAS9-HA-2xNLS-GFP
Insert Size (bp): 4975
Species: Synthetic; Streptococcus pyogenes
Fusion Proteins/Tags: Fusion gene Cas9-HA-2xNLS-GFP N/A
Vector Backbone and Size: pTREX-n, backbone size without insert: 6227bp
Cloning Site 5': XbaI
Cloning Site 3': HindIII
Antibiotic Resistance: Ampicillin
High or Low Copy: High
Grow in Standard E. coli @ 37C: DH5alpha
Selectable Markers: Neomycin (select with G418)
Storage Temperature: -20C
References
LanderN, Li ZH, Niyogi S, Docampo R. CRISPR/Cas9-Induced Disruption of ParaflagellarRod Protein 1 and 2 Genes in Trypanosomacruzi Reveals Their Role in Flagellar Attachment. mBIO. 2015.6(4): e01012-15.
- Country/Region
- USA
