Robust DNA Polymerase for PCR Application in Molecular Cloning and DNA Sequencing
Polymerase chain reaction (PCR) is a method for the rapid and exponential amplification of target nucleic acid sequences useful in gene characterization and molecular cloning technologies, including: the direct sequencing of PCR amplified DNA, the determination of allelic variation, and the detection of infectious and genetic disease disorders. Various thermostable DNA polymerases have been used for PCR applications; for example, Taq polymerase isolated from Thermus aquaticus, Pfu polymerase derived from Pyrococcus furiosus, KOD polymerase isolated from Thermococcus kodakaraensis, and Vent™ DNA polymerase isolated from Thermococcus litoralis. Although the current polymerases are effective and reliable, a potential market need exists for novel polymerases that are more robust than those currently available.
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