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Robust DNA Polymerase for PCR Application in Molecular Cloning and DNA Sequencing

詳細技術說明

Polymerase chain reaction (PCR) is a method for the rapid and exponential amplification of target nucleic acid sequences useful in gene characterization and molecular cloning technologies, including: the direct sequencing of PCR amplified DNA, the determination of allelic variation, and the detection of infectious and genetic disease disorders. Various thermostable DNA polymerases have been used for PCR applications; for example, Taq polymerase isolated from Thermus aquaticus, Pfu polymerase derived from Pyrococcus furiosus, KOD polymerase isolated from Thermococcus kodakaraensis, and Vent™ DNA polymerase isolated from Thermococcus litoralis. Although the current polymerases are effective and reliable, a potential market need exists for novel polymerases that are more robust than those currently available.

*Abstract

Researchers at KAUST have been seeking novel DNA polymerases from deep-sea brine pools of the Red Sea that could be useful molecular biology tools. With selective pressure in the Red Sea’s high salt environment, DNA polymerases are expected to be more tolerant of high salt and metal ion concentrations than commercially available polymerases. This can be useful when Pfu, Vent, and Taq fail due to a lack of ionic stability. The identified polymerase has been demonstrated to have robust reaction features of utilizing wide range of salt and metal ion concentration and metal ion types.

國家/地區

美國