B-05 Gene Delivery to Intact Plant Cell
ΓùÅ No pretreatment: CSCNT does not require pre-removal of cell walls. ΓùÅ No regeneration problem: using CSCNT does not result in the regeneration problem that is common in pre cell-wall-removal methods.ΓùÅ Versatility: CSCNT method can be applied to variety of plant cells.ΓùÅ High efficiency: CSCNT achieves high transfection efficiency as much as 6%, while conventional method, Silicon Carbide Fibers, realizes only 3%.
ΓùÅ Gene transfection to plant cellΓùÅ Gene delivery to hard-to-transfect cell
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Inventor: BABA, Yoshinobu | TOKESHI, Manabu | KAJI, Noritada | BAYOUMI, Ibrahim Maged Fouad Serag Eldin
Priority Number: WO2008156021A1
IPC Current: C12N001582 | C12N000510
Assignee Applicant: National University Corporation Nagoya University
Title: METHOD FOR INTRODUCING FOREIGN SUBSTANCE INTO CELL HAVING CELL WALL | PROCÉDÉ D'INTRODUCTION D'UNE SUBSTANCE ÉTRANGÈRE DANS UNE CELLULE AYANT UNE PAROI CELLULAIRE
Usefulness: METHOD FOR INTRODUCING FOREIGN SUBSTANCE INTO CELL HAVING CELL WALL | PROCÉDÉ D'INTRODUCTION D'UNE SUBSTANCE ÉTRANGÈRE DANS UNE CELLULE AYANT UNE PAROI CELLULAIRE
Summary: The method is useful for introducing foreign substance such as polynucleotide into a plant cell (claimed), where the foreign substance also includes antisense oligo DNA and RNA, plasmid DNA, proteins, enzymes, functional peptides, and oilgisaccharides.
Novelty: Method for introducing foreign substance into cell, by supplying carbon nanotube provided with enzyme, and foreign substance into treatment solution containing cell, decomposing cell wall, and introducing foreign substance into cell
生物医学
复康
美国
