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B-05 Gene Delivery to Intact Plant Cell

Technology Benefits

ΓùÅ No pretreatment: CSCNT does not require pre-removal of cell walls. ΓùÅ No regeneration problem: using CSCNT does not result in the regeneration problem that is common in pre cell-wall-removal methods.ΓùÅ Versatility: CSCNT method can be applied to variety of plant cells.ΓùÅ High efficiency: CSCNT achieves high transfection efficiency as much as 6%, while conventional method, Silicon Carbide Fibers, realizes only 3%.

Technology Application

ΓùÅ Gene transfection to plant cellΓùÅ Gene delivery to hard-to-transfect cell

Detailed Technology Description

None

Supplementary Information

Inventor: BABA, Yoshinobu | TOKESHI, Manabu | KAJI, Noritada | BAYOUMI, Ibrahim Maged Fouad Serag Eldin
Priority Number: WO2008156021A1
IPC Current: C12N001582 | C12N000510
Assignee Applicant: National University Corporation Nagoya University
Title: METHOD FOR INTRODUCING FOREIGN SUBSTANCE INTO CELL HAVING CELL WALL | PROCÉDÉ D'INTRODUCTION D'UNE SUBSTANCE ÉTRANGÈRE DANS UNE CELLULE AYANT UNE PAROI CELLULAIRE
Usefulness: METHOD FOR INTRODUCING FOREIGN SUBSTANCE INTO CELL HAVING CELL WALL | PROCÉDÉ D'INTRODUCTION D'UNE SUBSTANCE ÉTRANGÈRE DANS UNE CELLULE AYANT UNE PAROI CELLULAIRE
Summary: The method is useful for introducing foreign substance such as polynucleotide into a plant cell (claimed), where the foreign substance also includes antisense oligo DNA and RNA, plasmid DNA, proteins, enzymes, functional peptides, and oilgisaccharides.
Novelty: Method for introducing foreign substance into cell, by supplying carbon nanotube provided with enzyme, and foreign substance into treatment solution containing cell, decomposing cell wall, and introducing foreign substance into cell

Industry

Biomedical

Sub Category

Rehabilitation

*Abstract

Researchers at Nagoya University discovered a novel way to deliver genes to intact plant cells via Carbon Nanotube technology. Cup-Stack Carbon Nanotube (CSCNT: length ~2 micron meter and diameter ~100 nano meter) coated with cellulase (cell wall degrading enzyme) creates nano-holes in the cell walls; seeps into the interior of the cell; penetrates the nuclear membrane; and delivers genes into the cell nucleus. This method realizes high transfection efficiency as much as 6% without any major cell damage and can be applied to a variety of plant cells.

Country/Region

USA