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一种高保真靶标基因建库方法及其试剂盒


Technology Benefits

将常规的建库和捕获分开的步骤整合为一个流程,有效简化流程,减少操作过程中DNA的损失


Technology Application

基因检测


Detailed Technology Description

一种高保真靶标基因建库方法及其试剂盒,该方法包括磷酸化步骤,在模板分子的5’端修饰磷酸基团;退火延伸步骤,使用第一引物退火、延伸,得到靶核苷酸分子;第一测序接头连接步骤,将第一测序接头连接至靶核苷酸分子,获得第一测序接头连接产物;酶切步骤,切断第一引物,得到第一测序接头连接产物;双链末端修平步骤,切除单链序列,得到平末端第一测序接头连接产物;第二测序接头连接步骤,将第二测序接头连接至平末端第一测序接头连接产物,得到第二测序接头连接产物;扩增步骤,用第二引物、第三引物扩增第二测序接头连接产物,得到文库。本发明将常规的建库和捕获分开的步骤整合为一个流程,有效简化流程,减少操作过程中DNA的损失。


Type of Cooperation

已授权


Application Date

04/06/2024 00:00:00


Application No.

ZL 2021 1 0230543.2


Country/Region

Mainland China

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