Induced Stomatic Stem Cells
Reprogramming of somatic cells to neural stem cells provides a method to generate personalized stem cells with low cancer risk.
Method to generate personalized neural stem cells
Reduced cancer risk due to transient reprogramming
Cost-efficient reprogramming to neural stem cells due to short-cut
This method is qualified to produce autologous neural stem cells that proliferate indefinitely and are able to re-differentiate into functional neural cells. The technology therefore applies to the tissue regeneration of neural tissue and disease modelling, especially in the central nervous system.
The most obvious application of reprogramming cells from a differentiated to an embryonic-like status is the use in tissue regeneration. However, two key obstacles need to be overcome for clinical realization, i.e. risk of reprogrammed cells to develop neoplasiae as well as cumbersome and costly cell culture procedures. Therefore, it is imperative to develop cost-efficient methods with a lower the risk of cancer.
The present invention has solved this problem by using a modification of the originally described method. Here, the transcription factors Sox2, cMyc and Klf4 are exogenously and stably expressed, whereas Oct4 is introduced with an exogenous transient expression system.
Licensing
27/02/2013 00:00:00
EP20130707277 20130227
- international:
C12N5/079; C12N5/0793; C12N5/0797; C12N5/10
- cooperative:
C12N5/0619; C12N5/0622; C12N5/0623; C12N2500/25; C12N2500/44; C12N2500/46; C12N2501/11; C12N2501/115; C12N2501/235; C12N2501/392; C12N2501/602; C12N2501/603; C12N2501/604; C12N2501/606; C12N2506/08; C12N2506/13; C12N2506/1307; C12N2510/00
Patent application
4182
Germany
