The Brightest, Red-Shifted Luciferase-Luciferin Bioluminescent Pairs
These biocompatible, coenzyme free luciferase-luciferin pairs are able to emit orders of magnitude more light than common ones, while also being red-shifted, enabling the light to pass through thicker regions of mammalian tissue, enabling deeper and more reliable in vivo bioluminescence assays in medical applications
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20180057801
Background Bioluminescence imaging utilizes enzyme-substrate pairs to catalyze luminesence reactions, leading to photons at specific wavelengths using biocompatible reagents and without the need of excitation. Unfortunately, existing pairs have emissions orders of magnitude weaker than fluorescence due to low catalytic efficiency (such as Firefly Luciferase-Luciferin, or FLuc-D-luciferin). Other existing pairs such as NanoLuc-furimazine emit more photons than FLuc-D-luciferin, but mostly in the blue range, which is absorbed readily by mammalian tissues, limiting their usefulness for deep tissue imaging. Tech ID/UC Case 29032/2016-710-0 Related Cases 2016-710-0
USA
