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High--Throughput Flow Cytometry on Adherent Cells
Detailed Technology Description
This method offers a simple alternative to cumbersome protocols currently in use for preparing adherent cell lines for medium to highthroughput screening by flow cytometry. Routinely used protocols on adherent cells include various wash and centrifugation steps, which are not only time consuming and low-throughput but also result in cell number loss (in our experience, up to 85% in four washes). Our method offers lifting of adherent cells without removing media, eliminates wash steps, and prevents cell loss; thus allowing high-throughput flow cytometry on adherent cells in 96 and 384-well plates.
Country/Region
USA
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