SIMPLEx, an Amphipathic Protein Fusion Strategy for Making Water-soluble Integral Membrane Proteins

This invention describesa new method called SIMPLEx (Solubilization of IMPs with high Levelsof Expression), an in vivosolubilization method of Integral Membrane Proteins (IMPs) in functionallyrelevant conformations without the need for detergents or mutations to the IMPitself.

• Patentfiled in the U.S. US20170275343
• DarioMizrachi & al. (2015). Making water-soluble integral membrane proteins in vivo using an amphipathic proteinfusion strategy. Nature Communications 6:6826.DOI:10.1038/ncomms7826;
• DarioMizrachi & al. (2017). A water-soluble DsbB variant that catalyzesdisulfide-bond formation in vivo. Nature Chemical Biology 13, 1022–1028. doi:10.1038/nchembio.2409;
• 'Shield' gives tricky proteins a newidentity – By Anne Ju, April9, 2015;
• ‘Changing the identity of cellularenzyme spawns new pathway’By Tom Fleischman June 20, 2017.

USA