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Reagents and Methods for Assaying Protein Synthesis and Amino Acid Transport

Detailed Technology Description

Reagents and assays use in basic research and drug discovery. The reagents include stable, isolated ternary complexes, as well as tRNA and EF-tu labelled for FRET, smFRET, or flourophore quenching. The assays include protein sequencing, protein translation detection, and amino acid transport.

Others

Burnett BJ, et al (2013) Elongation factor Ts directly facilitates the formation and disassembly of the Escherichia coli elongation factor Tu-GTP-aminoacyl-tRNA ternary complex. J Biol Chem. 2013 May 10;288(19):13917-28.
Published PCT application WO2012139046

Country/Region

USA

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