Preparation Of Functional Homocysteine Residues In Polypeptides And Peptides
Uses easily handled, readily available reagents,Allows facile incorporation of a wide range of functional modificationsAllows formation of more stable, charge neutral functional polypeptide productsCharge neutral alkyl homocysteine derivatives: more biocompatible, better able to mimic various biological materials
Synthesis of thermoresponsive polypeptidesDrug delivery/carrierTherapeuticsFunctionalized protein coatingsScaffold for regenerative medicine
Researchers at UCLA have developed a novel method for efficient, chemoselective transformation of methionines in peptides and polypeptides into stable, functional homocysteine derivatives. This process allows facile incorporation of a wide range of functional modifications for different uses. An example of this method is the modification of polypeptides via the thioether groups naturally present in methionine or in S-alkyl cysteine residues.
State Of Development Successfully introduced azides, alkynes, sulfonates, phosphonates, carbohydrates, and amines to polypeptides Background Methods for selective conversion of natural amino acids in peptides, polypeptides and proteins into different functional residues are desirable for many areas, including chemical biology, materials science, and pharmaceuticals. The introduced functionality can provide probes for tracking, mimicking of post-translational modifications, or a means to adjust biological and physical properties of biomacromolecules. Thermoresponsive polypeptides possessing a lower critical solution temperature (LCST) respond to temperature in solution, especially in aqueous media. They have received much attention for a variety of applications such as stimulus-responsive assemblies, and as materials for potential use in medicine. In particular, short oligoethylene glycol (OEG) containing thermoresponsive polypeptides have been prepared using a variety of methods, using different core amino acid residues, and also with a wide range in number of ethylene glycol repeats and means of their attachment to different residues. In order to introduce functionality at unique sites, it is essential that the natural residues are present in low abundance, which has focused much attention on cysteine, methionine, and N-terminal residues. While many methods are available for chemoselective modification of highly nucleophilic cysteine, some are potentially limited by racemization and moderate yields. Thus, there is a need for new methods to convert natural amino acids into different functional residues, and a need for new thermoresponsive polypeptides. Related Materials “Chemoselective synthesis of functional homocysteine residues in polypeptides and peptides”. Gharakhanian, E. G.; Deming, T. J. Chem. Commun., 2016, 52, 5336-5339. Additional Technologies by these Inventors Tech ID/UC Case 27543/2016-579-0 Related Cases 2016-579-0
Gharakhanian, E. G., & Deming, T. J. (2016). Role of Side-Chain Molecular Features in Tuning Lower Critical Solution Temperatures (LCSTs) of Oligoethylene Glycol Modified Polypeptides. Journal of Physical Chemistry B, 120(26), 6096-6101.
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