A High-Throughput Biochemical Fluorometric Method for Measuring HDL Redox Activity

• More accurate indicator of cardiovascular risk• Inexpensive• Rapid• Scalable/high-throughput• Cell-free assay of HDL function

• Clinical heart disease diagnostic tool• Research heart disease diagnostic tool• HIV research• Autoimmune disease research

Dr. Theodoros Kelesidis, Dr. Otto Yang, and Dr. Srinivasa Reddy have invented a method of measuring the functionality of HDLs by measuring their redox activity using the Amplex Red fluorochrome. This reproducible assay has a 96 well format, making it inexpensive, rapid, and suitable for high throughput screening applications. This invention can be used both in cardiovascular disease research to better understand the relationship between HDL redox activity and arthrosclerosis, and in clinical settings as a tool for measuring cardiovascular risk in patients. This technology has the additional potential to be used in the research of many systemic inflammatory diseases such as AIDS and autoimmune diseases, as there are also studies that correlate dysfunctional HDL with HIVi and rheumatoid arthritisii. [i] Theodoros Kelesidis, Otto O. Yang, Judith S. Currier, Kaveh Navab, Alan M. Fogelman, and Mohamad Navab. HIV-1 infected patients with suppressed plasma viremia on treatment have pro-inflammatory HDL. Lipids in Health and Disease. 2011 10:35 [ii] Charles-Schoeman C, Lee YY, Grijalva V, Amjadi S, FitzGerald J, Ranganath VK, Taylor M, McMahon M, Paulus HE, Reddy ST. Ann Rheum Dis. 2012 Jul;71(7):1157-62.

20170059595

State Of Development

The assay has been fully developed and tested, with current work involving testing multiple patient populations and using the assay to study HDL function.

Background

Cardiovascular disease remains the number one cause of death for both men and women in the United States. According to a 2011 study by the Center for Disease Control, approximately 600,000 people die of heart disease in the United States every year, which is 1 in every 4 deathsi.HDLs and cardiovascular disease show an inverse correlation. However, growing evidence suggests that higher HDL levels are not always beneficial since the proteins can become dysfunctional. Current methods of HDL testing only indicate the overall levels in the blood and say nothing about their size, density, composition, and functionality. All of these properties are associated with atherosclerosis, and the ability to measure them could become a great tool for diagnosing cardiovascular risk.
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[i] KD Kochanek, JQ Xu, SL Murphy, AM Miniño, HC Kung. Deaths: final data for 2009. National vital statistics reports. 2011 60(3)

Related Materials

Theodoros Kelesidis, Judith S. Currier, Diana Huynh, David Meriwether, Christina Charles-Schoeman, Srinivasa T. Reddy, Alan M. Fogelman, Mohamad Navab, and Otto O. Yang A biochemical fluorometric method for assessing the oxidative properties of HDL. Journal of Lipid Research. 2011 52:(12) 2341-2351.

Tech ID/UC Case

23986/2014-425-0

Related Cases

2014-425-0

USA

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