Proteins That Fluoresce At Infrared Wavelengths Or Singlet Oxygen Upon Illumination
First genetically encoded labels that are excited by far-red light and fluoresce in the true infrared.First proteins that can generate singlet oxygen without exogenous small molecules at higher quantum yield than current systems (> 0.15 vs. 0.055 for ReAsh-Tetracystein).Easily expressed in mammalian cells.Easily excited by available and economical light sources and filter sets.Significantly less background from auto-fluorescence and without interference from most commonly used fluorophores.Singlet oxygen generation provides ability to detect protein-protein interactions over distances significantly greater than FRET systems (i.e. Tens of nm) and is independent of relative dipole orientation, usable in chromophore assisted light inactivation (CALI) and electron microscopy.Unrelated genetically and structurally to fluorescent proteins from Aequorea or Discosoma species.
Dr. Roger Tsien recently developed a new family of fluorescent proteins that emit infrared light. Unrelated both genetically and structurally to GFPs and RFPs, these variants are dimeric in nature but have been monomerized and have been developed from a single bacterial species. The proteins represent truncated variants of the full-length proteins, which do not exhibit infrared fluorescence. When the truncated protein is loaded with specific co-factors, they have the ability to fluoresce in the infrared or generate singlet oxygen without the addition of exogenous small molecules. The co-factors necessary for IR emission or singlet oxygen generation are available in most cell types and bind spontaneously. This is an ongoing project and new variants continue to be developed and tested.
Patent Number: US8653037B2
Application Number: US2010997548A
Inventor: Shu, Xiaokun | Royant, Antoine | Tsien, Roger
Priority Date: 11 Jun 2008
Priority Number: US8653037B2
Application Date: 23 Mar 2011
Publication Date: 18 Feb 2014
IPC Current: A61K003816
US Class: 5140212 | 4240096 | 435029 | 4353201 | 530324
Assignee Applicant: The Centre National de la Recherche Scientifique,Paris | The Regents of the University of California
Title: Proteins that fluoresce at infrared wavelengths or generate singlet oxygen upon illumination
Usefulness: Proteins that fluoresce at infrared wavelengths or generate singlet oxygen upon illumination
Summary: The isolated polynucleotide and methods are useful for in vivo optical imaging; and for generating singlet oxygen in cell, where the singlet oxygen is useful for determining protein-protein proximity or interaction or controlled photoablation of the protein or host cell (all claimed).
Novelty: New isolated polynucleotide encoding protein, e.g. infrared fluorescent protein (IFP) and chromophore-binding domain protein, useful for in vivo optical imaging and for generating singlet oxygen in cell
Biomedical
DNA/Gene Engineering
8653037
Related Materials Additional Technologies by these Inventors Tech ID/UC Case 22716/2008-303-0 Related Cases 2008-303-0
USA
