Quantitative Peptide Microarray Technology
By printing different sets of peptides on a microarray, this technology can be used for defining protein binding specificity, illustrating protein-protein interactions, determining epitopes recognized by antibodies, and identifying substrates of various enzymes.
Scientists at UC San Diego have developed a microarray technology providing a quantitative, high-throughput, and cost-effective way to conduct proteomics measurements. The present invention employs a microarray technology that prints peptides on a glass surface thereby significantly reducing the size of the array. It requires using fewer peptides than traditional methods, whereby thousands of peptides can be easily printed on one DNA microarray-sized slide, compared to the existing technology’s limit of only tens of peptides on the same surface area. This approach requires a small quantity of protein for analysis, which is particularly useful in disease diagnosis, where sample size is limited. Additionally, quantitative protein-peptide interactions (binding affinities) may be measured from fluorescence probe intensities.
Patent Number: US8728980B2
Application Number: US13666284A
Inventor: Wang, Wei | Xu, Zheng
Priority Date: 4 Nov 2011
Priority Number: US8728980B2
Application Date: 1 Nov 2012
Publication Date: 20 May 2014
IPC Current: C40B003004 | G01N003368
US Class: 506009 | 4350071
Assignee Applicant: The Regents of the University of California
Title: Peptide microarray and method of use
Usefulness: Peptide microarray and method of use
Summary: The peptide microarray is useful for epitope mapping; for drug screening or vaccine development; and, in combination with computational predictions, for identifying modified peptides, where the modified peptides are methylated or acetylated peptides corresponding to post-translational modification (PTM) proteins that regulate lysine methylation. The microarray and method are useful for identifying one or more binding peptides interacting with a protein of interest or its domain (all claimed). The vaccine may be used for autoimmune diseases and allergies.
Novelty: Peptide microarray useful for epitope mapping, for drug screening or vaccine development, and for identifying modified peptides, comprises a solid support surface and predicted unique binding peptides
Chemical/Material
Chemical/Material Application
8728980
Related Materials Xu Z, Hou T, Li N, Xu Y, Wang W. 2011. Proteome-Wide Detection of Abl1 SH3 Binding Peptides by Integrating Computational Prediction and Peptide Microarray. Mol Cell Proteomics. 2011 Oct 24. [Epub ahead of print] Additional Technologies by these Inventors Tech ID/UC Case 22220/2010-063-0 Related Cases 2010-063-0
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