Defined and Xeno-free Media for Feeder Free Conditions for Maintenance of Human Induced Pluripotent Cells (iPSCs) and Embryonic Stem Cells (hESCs)
Xeno-free: can be used for hESC and iPSC based therapies since it has no trace of an animal contamination. iPS cells show higher number of derived clones with both X chromosomes activated. And more importantly, and contrary to all media available, our media can sustain them in long-term maintenance. Defined: no lot to lot variation, thus better control over experimental variation. Tested on H9 and several iPSC clones, isolated from different individuals and patients. Substantially cost savings compared to currently available equivalent media.No adaptation from mouse embryonic fibroblast feeder cells.
Long-term maintenance of human embryonic stem or embryonic-like stem cells used in differentiation studies, scale up for drug screening or cell-based therapies.
A xeno-free and defined media is devised for the maintenance of hESCs and iPSC in an undifferentiated state in the absence of feeder cells or feeder conditioned medium, using MatrigelTM or a synthetic polymer matrix. By not using feeder cells, the medium in combination with defined coating substrate, such as Corning's synthetic matrix, can truly offer a xeno-free, and totally defined culture condition for hESC and hiPSC. Additionally, this medium was designed to be more cost attractive compared to the current ones on the market, and is the only clinical-grade media that can be used for hESC- and iPSC-based therapies. Contrary to products labeled as xeno-free currently in the market, this new medium has no trace of the animal contaminate known as sialic acid Neu5Gc (N-Glycolylneuraminic acid). Neu5Gc can be metabolically incorporated into the surface of any cultured cells. Most humans have antibodies to Neu5Gc from dietary sources such as milk and beef. The incorporation of nonhuman Neu5Gc into the cell surface of transplanted human cells will likely evoke undesirable immune responses from the host and compromise the efficacy and safety of cell based therapies. Moreover, the formulation can better buffer the variations caused by cellular metabolism, making the daily medium change unnecessary, saving on both cost and time. Finally, the serum replacement technology used in this medium was shown to increase the freeze-thaw efficiency in about 100-fold when compared to commercially available media.
Patent Number: US20130059377A1
Application Number: US13696286A
Inventor: Muotri, Alysson | Marinho, Paulo Andre Nobrega | Rehen, Stevens Kastrup | Dos Reis Castilho, Leda
Priority Date: 5 May 2010
Priority Number: US20130059377A1
Application Date: 5 Nov 2012
Publication Date: 7 Mar 2013
IPC Current: C12N0005071 | C12N00050735
US Class: 435366 | 435377
Title: STEM CELL DEFINED MEDIA FOR XENO-FREE AND FEEDER FREE CONDITIONS AND USES THEREOF
Usefulness: STEM CELL DEFINED MEDIA FOR XENO-FREE AND FEEDER FREE CONDITIONS AND USES THEREOF
Summary: The defined low protein culture medium is used for maintaining stem cells in an indifferentiated state (claimed).
Novelty: Defined low protein culture medium used for maintaining stem cells in an indifferentiated state, comprises a basal medium, an organic acid from the tricarboxylic acid cycle, nonessential amino acids, and growth factors
Biomedical
DNA/Gene Engineering
9725695
State Of Development Tested on several patient-derived iPSC clones and H9. Tested in different labs and by independent researchers. Related Case SD2010-299 Related Materials Tech ID/UC Case 19954/2010-036-0 Related Cases 2010-036-0
USA
