Methods of Tissue Repair, Regeneration, and Tissue Engineered Compositions
Potential uses of pleiotrophin and related factors include: Kidney growth, morphogenesis, and regeneration in vivo and ex vivo. In vitro engineering of nephrons and artificial kidneys from kidney progenitor cells and embryonic tissues.Repair and regeneration of the injured kidney (eg. acute tubular necrosis from ischemic or toxic insult).Protection and growth of tissue for transplantation.Treatment of chronic kidney by gene therapy or other method of administration.
Scientists at UC San Diego have developed a method of propagating ureteric bud (UB) cells in culture under conditions that induce the UB to undergo branching morphogenesis in order to generate a population of UB comprising tubular branches, subdividing the UB population, and resuspending each subpopulation in culture media. They have isolated an epithelial ureteric bud branching morphogenetic activity from the metanephric mesenchyme-derived cell conditioned medium and identified one of such molecules as an 18 kDa heparin binding protein, pleiotrophin. The invention demonstrates that purified pleiotrophin induces impressive branching morphogenesis of the isolated UB in vitro and provides methods of using pleiotrophin and compositions comprising pleiotrophin or other factors in the conditioned medium to induce morphogenesis in the kidney cells in vitro and in vivo. Additionally, methods and compositions are provided for constructing stable mammalian embryonic epithelial tissues and organs as well as constructing kidney tissue and treating renal failure.
Patent Number: US7326570B2
Application Number: US2003608783A
Inventor: Nigam, Sanjay Kumar | Sakurai, Hiroyuki | Bush, Kevin T.
Priority Date: 16 Jun 2000
Priority Number: US7326570B2
Application Date: 27 Jun 2003
Publication Date: 5 Feb 2008
IPC Current: A61K003818 | C12N0005071
US Class: 435369 | 4240931 | 4240937 | 435325 | 435375 | 435395 | 435405
Assignee Applicant: The Regents of the University of California
Title: Induction of tubular morphogenesis using pleiotrophin
Usefulness: Induction of tubular morphogenesis using pleiotrophin
Summary: (M1) is useful for propagating uteric bud (UB) cells in culture. (IV) is useful for treating a subject suffering from kidney failure, which involves transplanting (IV) into the subject. (I)-(IV) is useful for transplantation into a subject, where the tissue is implanted into a subject for the purpose of regeneration, due to ischemic or toxic insult or for the purpose of protection and growth of the tissue. (M10) is useful for treating acute renal failure (claimed).
Novelty: Propagating uteric bud (UB) cells in culture, by culturing UB in vitro to induce UB to undergo branching morphogenesis to generate UBs comprising tubular branches and subdividing UBs, resuspending UBs in media and repeating culturing
Disease Diagnostic/Treatment
Other Disease
7326570
Additional Technologies by these Inventors Tech ID/UC Case 19692/2001-205-0 Related Cases 2001-205-0
USA
