Determination of Protein Size
This invention teaches the preparation and use of porous Si films containing a controlled distribution of pore sizes for a unique bio-sensing application. Use of this invention to achieve the simultaneous separation and detection of a protein in a nano-machined silicon matrix is described. Gating of the response by adjustment of pH below and above the isoelectric point of the protein has also been demonstrated, and provides an additional means of bio-molecule separation and identification. This invention is useful for the determination of protein size and for the detection of weakly-bound complexes. In addition, the invention can controllably trap and release proteins from a microporous matrix and is useful for drug delivery applications, as porous Si has been shown to be bio-compatible and readily bio-resorbable.
Patent Number: US20130217786A1
Application Number: US13590825A
Inventor: Sailor, Michael J. | Abbi, Gaurav | Collins, Boycee | Dancil, Keiki-Pua S.
Priority Date: 5 Mar 2003
Priority Number: US20130217786A1
Application Date: 21 Aug 2012
Publication Date: 22 Aug 2013
IPC Current: C25F000314 | A61K000900 | C12M000134 | C12Q000100 | G01J000300 | G01J000344 | G01N002141 | G01N0033543 | G01N0033552
US Class: 514770 | 205665
Assignee Applicant: The Regents of the University of California
Title: POROUS NANOSTRUCTURES AND METHODS INVOLVING THE SAME
Usefulness: POROUS NANOSTRUCTURES AND METHODS INVOLVING THE SAME
Summary: The method is useful for simultaneously detecting and separating a target analyte such as biological cell, protein or other macromolecule, fragment of protein, polymer, or small molecule.
Novelty: Simultaneously detecting and separating target analyte, e.g. protein, by exposing porous silicon matrix to environment containing the analyte, observing optical reflectivity of matrix, and correlating changes in silicon substrate to analyte
Chemical/Material
Nano Materials
8274643
Related Materials Tech ID/UC Case 19561/2003-182-0 Related Cases 2003-182-0
USA
