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Method For High Level Production Of Recombinant Protein

Technology Application

The present invention provides methods for delivering higher yields than the Baker system. The invention is useful for the production of both soluble and membrane proteins. The technology produces proteins and peptides with authentic N termini for a range of downstream applications. Commercial applications include reagent uses, such as: Production of proteins in their native form Single or multiple amino acid mutants Isotopically labeled reagents Fluorescent or biotinylated reagents Reagents for protein therapeutics Animal testing reagents and/or components of kits

Detailed Technology Description

Scientists at the UC San Diego have designed a recombinant protein expression system that delivers significantly higher yields than previously reported systems and includes an easy means of purification. It allows the production of a variety of proteins and peptides with authentic N termini for a range of downstream applications. Higher yields are obtained by redesigned protein core amino acids because of reduced toxicity and proteolysis due to the "tuned" stability of the ubiquitin fusion. They are expressed in inclusion bodies that enable the proteins to be refolded and efficiently cleaved from the fusion by ubiquitinase. This results in higher expression levels than comparable systems that express soluble proteins. Ultimately, the present technology can be useful for the production of both soluble and membrane proteins.

Supplementary Information

Inventor: HANDEL, Tracy, M. | DOUGLAS, Andrew
Priority Number: WO2012129449A3
IPC Current: C07K001900 | C12N001562 | C12N001570
Assignee Applicant: The Regents of the University of California
Title: HIGH LEVEL PRODUCTION OF RECOMBINANT PROTEINS | PRODUCTION À HAUT NIVEAU DE PROTÉINES RECOMBINANTES
Usefulness: HIGH LEVEL PRODUCTION OF RECOMBINANT PROTEINS | PRODUCTION À HAUT NIVEAU DE PROTÉINES RECOMBINANTES
Summary: The method is used for producing a recombinant protein (claimed).

Industry

Chemical/Material

Sub Group

Chemical/Material Application

Others

State Of Development

A U.S. patent application has been filed.

Tech ID/UC Case

19155/2009-147-0

Related Cases

2009-147-0

Country/Region

USA

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