New Collision-Induced Dissociation Cross Linker and Related Software Package for Fast and Accurate Mass Spectrometry Analysis of Proteins
This new cross-linker allows for the MS analysis of multi-subunit protein complexes. In addition, the accompanying software package allows for the fast and accurate identification of DSSO-cross-linked peptides via MS.
None
Patent Number: US20130144541A1
Application Number: US13471365A
Inventor: Rychnovsky, Scott | Huang, Lan
Priority Date: 14 May 2011
Priority Number: US20130144541A1
Application Date: 14 May 2012
Publication Date: 6 Jun 2013
IPC Current: G01N003368 | H01J004900
US Class: 702020 | 435023 | 546117 | 548259 | 548406 | 548462 | 548520
Title: MASS SPECTROMETRY-CLEAVABLE CROSS-LINKING AGENTS TO FACILITATE STRUCTURAL ANALYSIS OF PROTEINS AND PROTEIN COMPLEXES, AND METHOD OF USING SAME
Usefulness: MASS SPECTROMETRY-CLEAVABLE CROSS-LINKING AGENTS TO FACILITATE STRUCTURAL ANALYSIS OF PROTEINS AND PROTEIN COMPLEXES, AND METHOD OF USING SAME
Summary: (I) is useful for mapping protein-protein interactions of protein complexes and for integrated data analysis workflow for identification of crosslinked peptides (all claimed).
Novelty: New mass spectrometry cleavable crosslinker having two symmetric collision induced dissociation cleavable sites, useful for integrated data analysis workflow for identification of crosslinked peptides
Biomedical
DNA/Gene Engineering
9222943
State Of Development This cross-linker and its accompanying software have been successfully used for the structural characterization of yeast 20 S proteasome complex and also demonstrated is identifying and characterizing model peptides and proteins. Tech ID/UC Case 21327/2010-689-0 Related Cases 2010-689-0
USA
