Microfabricated Capillary Electrophoresis Chip and Method for Simultaneously Detecting Multiple REDOX Labels
CE-EC chip designs are described which can be microfabricated so as to exploit the differences between redox-active labels for the simultaneous detection of multiple analytes during a separation.
Highly selective multiplex labeling and electrochemical detection of multiple analytesDNA sequencing
None
Patent Number: US6361671B1
Application Number: US1999229386A
Inventor: Mathies, Richard A. | Singhal, Pankaj | Xie, Jin | Glazer, Alexander N.
Priority Date: 11 Jan 1999
Priority Number: US6361671B1
Application Date: 11 Jan 1999
Publication Date: 26 Mar 2002
IPC Current: G01N0033483 | G01N0027416 | G01N0027447
US Class: 204452 | 204409 | 204603 | 210656 | 422070
Assignee Applicant: The Regents of the University of California
Title: Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
Usefulness: Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
Summary: For simultaneously detecting multiple redox-active labels.
Novelty: Microfabricated electrophoresis chip for simultaneous detection of multiple redox labels uses a matrix-coding scheme
Electronics
Semiconductor
Brief Description Capillary Electrophoresis (CE) is a powerful tool for DNA-sequencing and fragment sizing due to its low sample volume requirements, higher efficiency and rapidity of separations compared to the traditional approach of slab gel electrophoresis. The development of these miniaturized CE platforms has been driven by the concept of making fully integrated, inexpensive and portable analytical systems. Electrochemical (EC) detection is an approach that is easily adaptable to miniaturized CE platforms without any sacrifice in sensitivity or selectivity. EC detection has been widely used with conventional CE in fused-silica capillaries for highly sensitive and selective detection of various analytes. A critical problem in this application is figuring out how to decouple the high electrophoretic separation currents from the electrochemical detection system. UC Scientists have described a novel microfabricated capillary electrophoresis chip for detecting multiple redox-active labels simultaneously using a matrix coding scheme and a method of selectively labeling analytes for simultaneous electrochemical detection of multiple label-analyte conjugates after electrophoretic or chromatographic separation. Redox labels can be attached to all possible analytes such as DNA, RNA, nucleotides, peptides and proteins, carbohydrates and amino acids, etc. UC Scientist also described a method that simultaneously detects electrochemical signals generated at detection electrodes by different redox labels attached to analytes in a mixture of analytes, after the analytes have been separated in the electrophoresis chip. Additional Technologies by these Inventors Tech ID/UC Case 18627/1999-041-0 Related Cases 1999-041-0
USA
