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Quantitation and Characterization of Human Nuclear and Mitochondrial DNA


Summary

OSUresearchers have invented a unique PCR method, Quantitative TemplateAmplification Technology (Q-TAT), that simultaneously amplifies DNA, determinesthe amount of human nuclear and mitochondrial DNA present in a forensic sample,determines the gender of the DNA donor, assesses the quality of the DNA, and determineswhether or not the sample contains significant levels of PCR inhibitors thatwould interfere with amplification.


Technology Benefits

Highly sensitive and reproducibleEnables forensic DNA-typing labs to useexisting technology and instrumentationPermits simultaneous quantification of bothnuclear and mitochondrial DNALess expensive than current methods


Technology Application

Forensic analysis of DNA samples


Detailed Technology Description

None


Country/Region

USA

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