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Narrow I.D. Monolithic Capillary Columns for High Efficiency Separation and High Sensitivity Analysis of Biomolecules

Technology Benefits

Value PropositionThe column/method:ΓÇóIs highly cost effective, as it avoids use of expensive equipments such as high pressure pumps and special adaptersΓÇóEnables a high efficiency separation and a high sensitivity analysis of biomolecules as compared to conventional approachesΓÇóAllows for analytes to have a higher electrospray ionization abilityΓÇóCompletely avoids band broadening problems as observed with prior art approachesΓÇóMinimizes the monolith structural irregularities as associated with capillaries narrower than 50 ┬╡m IDΓÇóHas an extremely small internal diameter of ~25┬╡m or less as compared to conventional columnsΓÇóExhibits enhanced mass transfer properties with a low back pressureΓÇóExhibits an excellent column-column reproducibility as compared to conventional approachesΓÇóAllows for the analysis of biomolecules as low as one attomoleΓÇóWould be commercially useful for the following applications:o High mass sensitivityo Single or multi-dimensional nano-LC analysis of biological or clinical sampleso Effective coupling to separation/analytical techniques such as ESI-MS, MALDI-MSo Sample desalting prior to MS

Detailed Technology Description

None

Country/Region

USA

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