亞洲知識產權資訊網為知識產權業界提供一個一站式網上交易平台，協助業界發掘知識產權貿易商機，並與環球知識產權業界建立聯繫。無論你是知識產權擁有者正在出售您的知識產權，或是製造商需要購買技術以提高操作效能，又或是知識產權配套服務供應商，你將會從本網站發掘到有用的知識產權貿易資訊。

返回搜索結果

Single-Cell Isoelectric Focusing and pH Gradient Arrays

技術優勢: Highly-multiplexed isoelectric focusing separationsHigh-throughputCan be used for multiple sample typesStraightforward electrical interfacingSample loading can be automated

技術應用: Drug and vaccine developmentClinical diagnostics development and manufacturingProteomic instrument for commercial and research life science investigations

*Abstract: Post-translational protein modifications, such as glycosylation or phosphorylation, are the level at which this heterogeneity is expressed, and may serve as disease biomarkers. These modifications only result in small molecular mass changes, meaning that standard size-based separation techniques cannot be used. Isoelectric focusing (IEF) is a powerful technique that can resolve a single electrostatic charge difference between protein isoforms, and can be used for the charge-based separation post-translationally modified proteins require. Multiplexed IEF separations are challenging due to the unique chemical environment needed. Although IEF would be ideal for single-cell protein measurement, current techniques rely on specific antibodies to resolve different isoforms, limiting the assays in scope.

Researchers at UC Berkeley have addressed these issues by developing a single-cell isoelectric focusing technique that uses pH gradient arrays. By multiplexing IEF, this technology enables separation of the contents of single cells to get insights into cell heterogeneity while retaining the analytical performance to resolve small charge differences.

*Principal Investigation: Name: Amy Herr
Department:

Name: Augusto Tentori
Department:

其他

Additional Technologies by these Inventors

Tech ID/UC Case

25107/2015-192-0

Related Cases

2015-192-0

欲了解更多信息，請點擊這裡