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Single-Cell Isoelectric Focusing and pH Gradient Arrays

技术优势: Highly-multiplexed isoelectric focusing separationsHigh-throughputCan be used for multiple sample typesStraightforward electrical interfacingSample loading can be automated

技术应用: Drug and vaccine developmentClinical diagnostics development and manufacturingProteomic instrument for commercial and research life science investigations

*Abstract: Post-translational protein modifications, such as glycosylation or phosphorylation, are the level at which this heterogeneity is expressed, and may serve as disease biomarkers. These modifications only result in small molecular mass changes, meaning that standard size-based separation techniques cannot be used. Isoelectric focusing (IEF) is a powerful technique that can resolve a single electrostatic charge difference between protein isoforms, and can be used for the charge-based separation post-translationally modified proteins require. Multiplexed IEF separations are challenging due to the unique chemical environment needed. Although IEF would be ideal for single-cell protein measurement, current techniques rely on specific antibodies to resolve different isoforms, limiting the assays in scope.

Researchers at UC Berkeley have addressed these issues by developing a single-cell isoelectric focusing technique that uses pH gradient arrays. By multiplexing IEF, this technology enables separation of the contents of single cells to get insights into cell heterogeneity while retaining the analytical performance to resolve small charge differences.

*Principal Investigation: Name: Amy Herr
Department:

Name: Augusto Tentori
Department:

其他

Additional Technologies by these Inventors

Tech ID/UC Case

25107/2015-192-0

Related Cases

2015-192-0

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