亚洲知识产权资讯网为知识产权业界提供一个一站式网上交易平台，协助业界发掘知识产权贸易商机，并与环球知识产权业界建立联系。无论你是知识产权拥有者正在出售您的知识产权，或是制造商需要购买技术以提高操作效能，又或是知识产权配套服务供应商，你将会从本网站发掘到有用的知识产权贸易资讯。

返回搜索结果

Real-Time Detection of Label-Free Protein Adsorption

总结: Researchers at Purdue University have developed a method for the novel use of nonlinear optical null ellipsometry for background-free, real-time biosensing of protein/surface interactions. Adsorption of bovine serum albumin (BSA) at silica/aqueous solution interfaces was used to induce a change in the polarization state of frequency-doubled light. This change in polarization is translated through weak, dynamic interactions with a co-absorbed nonlinear optical probe molecule. Signals arising from surface interactions with BSA were spatially isolated and selectively detected during BSA adsorption and confirmed conformational changes within the protein layer, consistent with denaturation. This technology allows for chiral-specific detection in small sample sizes with unprecedented sensitivity to chirality and requiring relatively simple instrumentation. Chiral synthesis, separation, and detection are hot topics and current optical detection methods are not particularly sensitive to chirality.

技术优势: Greater flexibility of selectionBetter retention and content of phase information Nonlinear sensitivity to changes

*Background: Ellipsometry is a well-established technique that is used in a number of industrial applications. Second harmonic generation (SHG) surface selectivity can be coupled with the reasonably high sensitivity of simple instrumentation to biosensing applications. Recent developments in real-time optical sensing approaches present attractive alternatives to other optical-based sensing techniques to in situ detection of unlabeled proteins.

*Web Links: Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipGarth SimpsonThe Simpson Research GroupPurdue Chemistry

欲了解更多信息，请点击这里