亞洲知識產權資訊網為知識產權業界提供一個一站式網上交易平台，協助業界發掘知識產權貿易商機，並與環球知識產權業界建立聯繫。無論你是知識產權擁有者正在出售您的知識產權，或是製造商需要購買技術以提高操作效能，又或是知識產權配套服務供應商，你將會從本網站發掘到有用的知識產權貿易資訊。

返回搜索結果

Real-Time Detection of Label-Free Protein Adsorption

總結: Researchers at Purdue University have developed a method for the novel use of nonlinear optical null ellipsometry for background-free, real-time biosensing of protein/surface interactions. Adsorption of bovine serum albumin (BSA) at silica/aqueous solution interfaces was used to induce a change in the polarization state of frequency-doubled light. This change in polarization is translated through weak, dynamic interactions with a co-absorbed nonlinear optical probe molecule. Signals arising from surface interactions with BSA were spatially isolated and selectively detected during BSA adsorption and confirmed conformational changes within the protein layer, consistent with denaturation. This technology allows for chiral-specific detection in small sample sizes with unprecedented sensitivity to chirality and requiring relatively simple instrumentation. Chiral synthesis, separation, and detection are hot topics and current optical detection methods are not particularly sensitive to chirality.

技術優勢: Greater flexibility of selectionBetter retention and content of phase information Nonlinear sensitivity to changes

*Background: Ellipsometry is a well-established technique that is used in a number of industrial applications. Second harmonic generation (SHG) surface selectivity can be coupled with the reasonably high sensitivity of simple instrumentation to biosensing applications. Recent developments in real-time optical sensing approaches present attractive alternatives to other optical-based sensing techniques to in situ detection of unlabeled proteins.

*Web Links: Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipGarth SimpsonThe Simpson Research GroupPurdue Chemistry

欲了解更多信息，請點擊這裡