RNA-Directed DNA Editing in Stem Cells
- 技术优势
- • Simple and time-efficient • User-friendly
- 详细技术说明
- Simple, efficient and user-friendly approach for DNA editing in mammalian cells #genomics #RNA #DNA #researchtool #DNA/RNAsynthesis
- *Abstract
-
Northwestern researchers have developed a method to accomplish genome editing in human pluripotent stem cells (hPSCs). This method uses ribonucleic acids (RNAs) as a guide for targeting genes of interest. Current methods are cumbersome and time-consuming. They require that a pair of proteins be designed, expressed and validated for each gene that is to be edited.Prof. Sontheimer and his colleagues have developed a simple and fast genome editing method that not only requires less reagents but also exhibits higher targeting efficiencies. This new technology offers a useful tool to discovery more stem cell-based therapies.
- *Inventors
- Alfonso Mondragon Eric Sontheimer* Yan Zhang Rakhi Rajan Zhonggang Hou James Thomson
- *Publications
- Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA (2013) Efficient Genome Engineering in Human Pluripotent Stem Cells Using Cas9 from Neisseria meningitidis, PNAS, 110: 15644-9.
- 国家/地区
- 美国
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