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RNA-Directed DNA Editing in Stem Cells

Technology Benefits: • Simple and time-efficient • User-friendly

Detailed Technology Description: Simple, efficient and user-friendly approach for DNA editing in mammalian cells #genomics #RNA #DNA #researchtool #DNA/RNAsynthesis

*Abstract: Northwestern researchers have developed a method to accomplish genome editing in human pluripotent stem cells (hPSCs). This method uses ribonucleic acids (RNAs) as a guide for targeting genes of interest. Current methods are cumbersome and time-consuming. They require that a pair of proteins be designed, expressed and validated for each gene that is to be edited.Prof. Sontheimer and his colleagues have developed a simple and fast genome editing method that not only requires less reagents but also exhibits higher targeting efficiencies. This new technology offers a useful tool to discovery more stem cell-based therapies.

*Inventors: Alfonso Mondragon Eric Sontheimer* Yan Zhang Rakhi Rajan Zhonggang Hou James Thomson

*Publications: Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA (2013) Efficient Genome Engineering in Human Pluripotent Stem Cells Using Cas9 from Neisseria meningitidis, PNAS, 110: 15644-9.

Country/Region: USA

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