TM4 Conditional Shuttle Plasmids and Uses Thereof
- 详细技术说明
- None
- *Abstract
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The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the TM4 mycobacteriophage that introduces DNA of interest into mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a TM4 conditional shuttle phasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and a method of generating auxotrophic mutants.
- *Principal Investigation
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Name: Stoyan Bardarov
Department:
Name: Graham Hatfull
Department: Distinguished Faculty-Dietrich School of Arts and Sciences
Name: William Jacobs, Jr, Investigator and Professor
Department: Department of Microbiology and Immunology
- 国家/地区
- 美国
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