Anti-(rat)-and Anti -(human)-mannosidase (II) polyclonal antibodies.
- Detailed Technology Description
- Applications α-mannosidase II is a hydrolase involved in the processingof asparagine-linked oligosaccharides, is an integral membrane glycoproteinfacing he lumen of Golgi membranes. The enzyme is also a member of a growingclass of Golgi enzymes that contain globular catalytic domains anchored to thelumen of the Golgi complex by a terminal membrane-spanning domain. Man IImodifies the GIcNAcMansGlcNAc2 intermediate to complete the mannose trimmingreactions and gives rise to the endoglycosidase H (endo H)-resistant oligosaccharide,GIcNAcMan3GlcNAc2. Golgi mannosidase II (GMII) is a core component of theN-glycosylation pathway. GMII inhibitors are of interest as potentialanti-cancer or anti-infection compounds. The antibody was raised to rat Golgialpha-mannosidase II purified from rat liver by standard chromatographyapproaches. The protein was emulsified with Freunds complete adjuvant andimmunized in rabbits to generate a polyclonal antiserum. Recommended for use inimmunofluorescence.
- *Abstract
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Reagent Description
Antigen:
Rat Golgi alpha-mannosidase II
Accession ID:
NCBI Ref.Seq.: NP_037111, Uniprot P28494
Molecular Weight:
~110 kDa
Clonality:
Polyclonal
Reactivity:
Rat, human and possibly others
Immunogen:
Purified protein
Species Immunized:
Rabbit
Buffer:
Rabbit Serum
Tested Applications:
IF
References
Moremen,K. W., O. Touster, and P. W. Robbins. 1991. Novel purification of the catalyticdomain of Golgi α-mannosidase II. Characterization and comparison with theintact enzyme. J. Biol. Chem.266:16876-16885.
Velasco,A., Hendricks, L., Moremen, K.W., Tulsiani, D.R., Touster, O., and Farquhar,M.G. (1993) Cell type–dependent variations in the subcellular distribution ofalpha-mannosidase I and II. J. CellBiol. 122: 39–51
K.W.Moremen. 2001. a-Mannosidase II. In: Handbook of Glycosyltransferases andRelated Genes (N. Taniguchi and M. Fukuda, eds.), pp. 600-606. Springer-Verlag,Tokyo.
- Country/Region
- USA
