Monoclonal antibody panel against surface epitopes of several strains of M. gallisepticum (10 lines)
- Detailed Technology Description
- ApplicationsThese10 IgG monoclonal antibodies were generated against various strains of Mycoplasma gallisepticum and recognize M. gallisepticum surface epitopes, andtheir use has been validated for ELISA, DIBA, immunoblotting, immunoperoxidaseassay and Haemagglutination Inhibition applications, and have the potential tobe used in therapeutic measures.Mycoplasmagallisepticum (Mg) is a bacterium belonging to the class Mollicutes and thefamily Mycoplasmataceae. It is thecausative agent of chronic respiratory disease (CRD) in chickens and infectioussinusitis in turkeys, chickens, game birds, pigeons, and passerine birds,causing some level of mortality. Once infected, they are carriers for thedisease for life. Since the disease causes reduced feed and growth production,carcass condemnations, and retarded growth in juveniles, serious economiclosses have occurred. Also, chickens have been documented to lose about 16 eggsover their laying cycle of 45 weeks. Mycoplasma lack cell walls, have highlyvariable surface proteins and a distinctive plasma membrane, and are thesmallest self-replicating prokaryotes. Mycoplasma can cause disease in humans,animals, insects, and plants. Isolates of Mycoplasmagallisepticum vary widely in their pathogenicity depending on the nature ofthe isolate, its method of propagation, and the number of passages. Among Mgstrains significant differences have also been observed in their tissuetropism, transmission and in the immune response they induce.
- *Abstract
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Reagent Description
Antigen:
Surface epitopes of M. gallisepticum
Molecular Weight:
56-58 [and 79, strain dependent]
Clone names:
Isotype:
8F7.A5, 4G1.B1, 9D4.G1, 12D10.H5, 6A10.H6, 3D6.C6, 12D8.H9, 3E10.E9, 4B12.A8, 7G1.A8
IgG1 (all)
Clonality:
Monoclonal
Clone Name:
8F7.A5
Reactivity:
M. gallisepticum
Immunogen:
Strain F
Species Immunized:
Mouse
Purification Method:
Protein G
Buffer:
0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications:
ELISA, DIBA, Immunoblotting, Immunoperoxidase assay and Haemagglutination inhibition
Storage:
-20C
12D10.H5 and 6A10.H6 exhibited similar inhibitingpotency against 4 HA units of five different Mg strains. Partial HI wasobserved at Mab concentration from 20 to 50 µg/mL, while 4 to 16 times higherconcentration yielded complete HI, but appear not to inhibit haemadsorption.
3D6.C6reacts strongly with p98 protein (98 kDa, PI=5.2) and with F, R, S6, A5969 andts11 strains, and does not react with M.synoviae or M. iowae, and verylittle/no reaction is observed against 6/85 membrane. 3D6.C6 has potential for serodiagnostic development.
References
Bencina D,Kleven SH, Elfaki MG, Snoj A, Dovc P, Dorrer D, Russ I. Variable expression ofepitopes on the surface of Mycoplasma gallisepticum demonstrated withmonoclonal antibodies. Avian Pathol.1994 Mar;23(1):19-36.
García M, ElfakiMG, Kleven SH. Analysis of the variability in expression of Mycoplasmagallisepticum surface antigens. Vet Microbiol.1994 Nov;42(2-3):147-58.
Elfaki, MG,Kleven SH, Ragland, WL. Identification of specie-specific glycoprotein on thesurface of Mycoplasma gallisepticum. FASEB. J., 5: A516.
Avakian AP,Kleven SH, Ley DH. Comparison of Mycoplasma gallisepticum strains andidentification of immunogenic integral membrane proteins with Triton X-114 byimmunoblotting. Vet Microbiol.1991 Nov;29(3-4):319-28.
Elfaki MG,Kleven SH, Ragland WL, Steffens WL, Blankenship LL. Evidence for a common epitopeon the surface of Mycoplasma gallisepticum defined by monoclonal antibody. Vet Microbiol.1993 May;35(1-2):161-77.
- Country/Region
- USA
