Next Generation Bovine Diagnostic Panel
- *Abstract
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NextGeneration Bovine Diagnostic Panel
Applications
· Multiplexdiagnostic panel for bovine infectious disease
· Detectsthe presence of 45 of the most common pathogens
· Amultiplicity of sample types can be used, including tissues, blood, milk andswabs
ProblemsAddressed
· Moresensitive and specific than traditional culture-based diagnostics
· Canscreen a sample for multiple pathogens simultaneously
· Easydata analysis
· Fast
· Inexpensive
PathogensValidated
Respiratory Pathogens
Enteric Pathogens
Repro Pathogens
Mastitis
BVDV (with typing), other pestiviruses, IBR (vs vaccine strain), Bovine Coronavirus, Mycoplasma species, Influenza D, PI3, BRSV, Adenovirus 3, Histophilus somni, Pasturella multocida, Mannheimia haemolytica, Trueperella pyogenes, Bibersteinia trehalosi
E. coli toxins (F41, F5, sta, stx 1,2, eae, cnf 1/2, alpha hly) Salmonella, Rotavirus (A,B,C), Johne’s, Clostridium perfringens toxin typing (alpha, beta, beta2, epsilon, iota, cpe), Giardia, Cryptosporidium, Coronavirus
Neospora, Leptospirosis species, Toxoplasma, Chlamydia species, Campylobacter fetus fetus and venerealis, BHV-4, IBR, BVDV, Brucella abortus,
T. foetus, Bluetongue/EHD, Anaplasma marginale, Listeria monocytogenes, Ureaplasma
Staphylococcus aureus, E. coli toxins, Streptococcus agalactiae, Mycoplasma bovis, Prototheca, Streptococcus uberis, Streptococcus dysgalactiae,
Coag negative Staphylococcus, Pseudomonas, Klebsiella, Zygomycetes, Aspergillus, Nocardia
TechnologySummary
Diagnosis of infectiousdisease in cattle can be challenging, especially when the animal is infectedwith multiple pathogens. Traditionally infectious disease is diagnosed via thedetection of organisms by bacterial culture, virus isolation, or antibody-basedtechniques. These methodologies have limitations, including the need forspecialized staff, inefficiency in growth of cultures and need for propersample handling.
The advantages of a nucleicacid-based technique, such as PCR, are numerous and include speed, sensitivityand specificity. However, PCR has its own challenges in that it is limited bypathogens that can be detected in a single reaction. To address that concern,UGA researchers have developed a Next Generation Sequencing (NGS)-baseddiagnostic panel that is able to detect the presence of 45 different pathogensusing target-specific primers for PCR-mediated amplification. This multiplexpanel has been validated using clinical samples that were subsequently testedwith commonly used diagnostic techniques. These results confirm the validity of using NGS-based techniques inveterinary diagnostics.
Inventors
• Rebecca,Wilkes, DVM, PH.D., DACVM
Assistant Professor, Department of InfectiousDiseases
· EmanAnis, Ph.D.
Post-Doctoral Fellow, Department of InfectiousDiseases
TechnologyDevelopment and IP Status
· Primershave been developed and validated
· Testhas proven to be efficacious in clinical samples
· Manuscriptsubmitted for publication
· Technologyavailable for know-how/trade secret license
- Country/Region
- USA
