Dendrimer Modified Oligonucleotides for High Yield Singly Modified Nanoparticle Probes
- Detailed Technology Description
- The subject invention circumvents the use of statistical modifications by applying a proprietary process to grow nanoparticles directly on a protected DNA strand during the final stages of oligonucleotide synthesis.
- Others
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Additional Technologies by these Inventors
- Self-Assembling 2D and 3D Nanostructures for Nano-Photonic and Nano-Electronic IC's and Devices
- Microarray for High Throughput Detection of Enzymatic Activity
- DNA Double-Write/Double Binding Identity For Micro/Nano Lithography and Self-Assembly Nanofabrication
Tech ID/UC Case
19736/2007-037-0
Related Cases
2007-037-0
- *Abstract
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Known methods for creating nanoparticle DNA probes rely upon synthesis of a nanoparticle followed by a functionalization step to attach the DNA. DNA is generally attached to the nanoparticle through a variety of coupling chemistries (-NH, SH, hydrazides, biotin/streptavidin, etc.). Stoichiometric additions of a 1:1 mixture of DNA to nanoparticle will not result in singly modified nanoparticles because Poission statistics govern the interactions between nanoparticle and DNA. The expected value (average) of the Poisson distribution will generate one DNA per nanoparticle, but depending upon the available coupling regions on the nanoparticle, a large percentage of nanoparticles will carry many more DNA molecules. For precise nanostructure assembly, singly modified probes need to be purified from the un and multiply-modified nanoparticles, leading to a very costly, low yield process for high purity nanoparticle probes. The problem becomes more pronounced as nanoparticle size increases, as the heterogeneity of nanoparticles make purification very difficult for oligonucleotide probes.
- *Principal Investigator
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Name: Michael Heller
Department:
Name: Xiaohua Huang
Department:
Name: Benjamin Sullivan
Department:
- Country/Region
- USA
